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Automated DNA array image segmentation and analysis

  • US 6,674,882 B1
  • Filed: 11/16/2001
  • Issued: 01/06/2004
  • Est. Priority Date: 02/07/1998
  • Status: Expired due to Term
First Claim
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1. A method for segmentation of a frame of image information including a plurality of spaced DNA spot images corresponding to a plurality of DNA spots, said image information including image intensity level information corresponding to said DNA spots, the method comprising the steps of:

  • (a) storing the frame of image information in memory;

    (b) selecting a set of image information within said frame including a selected set of the DNA spot images;

    (c) generating a grid in memory, the grid including a plurality of spaced grid points corresponding to said selected set of DNA spot images, the grid points having a predefined relationship, each grid point including position information indicating the position of the grid point within said image frame;

    (d) segmenting the selected set of image information by selecting at least one image segment defining a segment area around a grid point and including a spot image; and

    (e) quantifying at least a portion of image information in said image segment to obtain image characteristic values for said image segment, wherein the frame of image information includes a plurality of pixels each having an intensity level, and wherein the step of quantifying includes;

    (i) sorting at least a portion of the pixel intensities within said image segment, (ii) selecting a portion of said pixels, and (iii) computing an image characteristic value for the selected pixel values as function of the intensities of at least a portion of the selected pixel values.

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