Patterned substrates and methods for nerve regeneration
First Claim
Patent Images
1. A method for regenerating a severed nerve, comprising:
- providing a substrate having a surface containing one or more substantially linear grooves;
placing one or more guidance factors for nerve regeneration into said one or more grooves;
wherein said one or more guidance factors comprises poly(vinyl alcohol);
positioning said substrate in proximity to a severed end of said nerve such that said one or more grooves is substantially coextensive to said severed end; and
allowing said severed nerve to grow into said one or more grooves of said substrate.
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Accused Products
Abstract
Micropatterned substrates and methods for fabrication of artificial nerve regeneration conduits and methods for regenerating nerves are provided. Guidance compounds or cells are seeded in grooves formed on the patterned substrate. The substrates may also be provided with electrodes to provide electrical guidance cues to the regenerating nerve. The micropatterned substrates give physical, chemical, cellular and/or electrical guidance cues to promote nerve regeneration at the cellular level.
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Citations
75 Claims
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1. A method for regenerating a severed nerve, comprising:
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providing a substrate having a surface containing one or more substantially linear grooves;
placing one or more guidance factors for nerve regeneration into said one or more grooves;
wherein said one or more guidance factors comprises poly(vinyl alcohol);
positioning said substrate in proximity to a severed end of said nerve such that said one or more grooves is substantially coextensive to said severed end; and
allowing said severed nerve to grow into said one or more grooves of said substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. A method for regenerating a severed nerve, comprising:
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providing a porous guidance conduit having an inner surface and an outer surface;
providing a substrate containing one or more substantially linear grooves, wherein said one or more grooves contain one or more guidance factors for nerve regeneration and wherein said substrate is disposed on the inner surface of said conduit;
positioning said guidance conduit in proximity to a severed end of said nerve; and
allowing said severed nerve to grow into said grooves of said substrate. - View Dependent Claims (35)
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36. A method for regenerating a severed nerve, comprising:
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providing a substrate having a surface containing a plurality of substantially linear grooves, wherein said one or more grooves are substantially parallel and contain one or more guidance factors for nerve regeneration;
wherein said substrate comprises a material selected from the group consisting of poly(D,L-lactide), and copolymers of lactic and glycolic acids;
providing a porous guidance conduit having an inner surface wherein said substrate is disposed on the inner surface of said conduit;
attaching said guidance conduit between the severed ends of said nerve; and
allowing said severed nerve to grow into said grooves of said substrate. - View Dependent Claims (37, 38, 39)
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40. An apparatus for regenerating a severed nerve comprising:
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a substrate having a surface containing one or more substantially linear grooves at least about 5 μ
m wide, spaced at least about 10 μ
m apart, and at least about 1 μ
m deep, wherein said one or more grooves contain one or more guidance factors for nerve regeneration, said guidance factors being selected from the group consisting of Schwann cells, stem cells, nerve growth factor, laminin, collagen, polylysine and chicken plasma; and
one or more negative guidance factors comprising poly(vinyl alchohol). - View Dependent Claims (41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68)
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69. An apparatus adapted for connection to at least one end of a severed nerve, comprising:
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a cylindrical porous guidance conduit having an inner and an outer surface;
a substrate having a surface containing one or more substantially linear grooves, wherein said grooves contain one or more guidance factors for nerve regeneration; and
wherein said substrate is disposed on the inner surface of said guidance conduit.
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70. An apparatus adapted for connection to at least one end of a severed nerve, comprising:
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a cylindrical guidance conduit having an inner and an outer surface;
wherein said guidance conduit is sutured to at least one end of said severed nerve;
a substrate having a surface containing one or more substantially linear grooves, wherein said grooves contain one or more guidance factors for nerve regeneration; and
wherein said substrate is disposed on the inner surface of said guidance conduit.
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71. An apparatus for regenerating a severed nerve, comprising:
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a porous cylindrical guidance conduit having an inner and outer surface;
a substrate having a surface containing one or more substantially linear grooves, wherein said one or more grooves contain one or more guidance factors for nerve regeneration, said guidance factors being selected from the group consisting of Schwann cells, stem cells, nerve growth factor, laminin, collagen, polylysine and chicken plasma; and
wherein said substrate is disposed on the inner surface of said guidance conduit. - View Dependent Claims (72, 73, 74, 75)
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Specification