Functionalized silicon surfaces, and method for their production
First Claim
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1. A method for immobilising a bio-molecule on a silicon substrate, the method comprising the steps:
- (A) providing Si—
H groups at a surface of the silicon substrate;
(B) attaching a bi-functional linker molecule having a coupling group that is unreactive with said silicon substrate and with bio-molecules until activation or deprotection and a CH2═
CH—
group to the silicon substrate by reacting a Si—
H group at said surface with the CH2═
CH—
group of the linker molecule to form a covalent Si—
C bond between said silicon substrate and said linker molecule, thus forming an attached linker molecule;
(C) modifying said coupling group of said attached linker molecule by activation or deprotection to make said coupling group ready for reaction with a bio-molecule to form an attached linker molecule having an reactive coupling group; and
(D) covalently reacting said attached linker molecule having a reactive coupling group with a bio-molecule selected from the group consisting of a DNA, RNA, protein and carbohydrate, to immobilise said bio-molecule on said silicon substrate.
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Abstract
Provided is a method for modifying silicon with an organic molecule, under mild conditions. If the attached molecule is bi-functional, it may be subsequently reacted with a bio-molecule, to form a covalently attached layer of bio-molecule on the silicon surface.
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Citations
6 Claims
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1. A method for immobilising a bio-molecule on a silicon substrate, the method comprising the steps:
-
(A) providing Si—
H groups at a surface of the silicon substrate;
(B) attaching a bi-functional linker molecule having a coupling group that is unreactive with said silicon substrate and with bio-molecules until activation or deprotection and a CH2═
CH—
group to the silicon substrate by reacting a Si—
H group at said surface with the CH2═
CH—
group of the linker molecule to form a covalent Si—
C bond between said silicon substrate and said linker molecule, thus forming an attached linker molecule;
(C) modifying said coupling group of said attached linker molecule by activation or deprotection to make said coupling group ready for reaction with a bio-molecule to form an attached linker molecule having an reactive coupling group; and
(D) covalently reacting said attached linker molecule having a reactive coupling group with a bio-molecule selected from the group consisting of a DNA, RNA, protein and carbohydrate, to immobilise said bio-molecule on said silicon substrate. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification