Functionalized silicon surfaces, and method for their production

• US 6,677,163 B1
• Filed: 11/16/2000
• Issued: 01/13/2004
• Est. Priority Date: 11/16/2000
• Status: Expired due to Fees

• Current Assignee: National Research Council Canada
• Original Assignee: National Research Council Canada
• Inventors: Wojtyk, James, Boukherroub, Rabah, Wayner, Danial D. M.
• Primary Examiner(s): Geperley, Mary E.
• Application Number:
  US09/713,300
• Time in Patent Office: 1,153 Days
• Field of Search: 436/527, 436/823, 530/391.1, 530/402, 530/408, 530/409, 530/410, 435/6, 435/181
• US Class Current: 506/17
• CPC Class Codes: C07H 15/26 Acyclic or carbocyclic radicals, substituted by hetero rings
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/552 Glass or silica
• CPC Class Codes: Y10S 436/823 Immunogenic carrier or carrier per se

Abstract

Provided is a method for modifying silicon with an organic molecule, under mild conditions. If the attached molecule is bi-functional, it may be subsequently reacted with a bio-molecule, to form a covalently attached layer of bio-molecule on the silicon surface.

Claims

1. A method for immobilising a bio-molecule on a silicon substrate, the method comprising the steps:

(A) providing Si—

H groups at a surface of the silicon substrate;

(B) attaching a bi-functional linker molecule having a coupling group that is unreactive with said silicon substrate and with bio-molecules until activation or deprotection and a CH₂═

CH—

group to the silicon substrate by reacting a Si—

H group at said surface with the CH₂═

CH—

group of the linker molecule to form a covalent Si—

C bond between said silicon substrate and said linker molecule, thus forming an attached linker molecule;

(C) modifying said coupling group of said attached linker molecule by activation or deprotection to make said coupling group ready for reaction with a bio-molecule to form an attached linker molecule having an reactive coupling group; and

(D) covalently reacting said attached linker molecule having a reactive coupling group with a bio-molecule selected from the group consisting of a DNA, RNA, protein and carbohydrate, to immobilise said bio-molecule on said silicon substrate.

2. The method of claim 1, wherein the covalent bond to the surface is formed in a thermal reaction.

3. The method of claim 1, wherein the covalent bond to the surface is formed in a photochemical reaction.

4. The method of claim 1, wherein the silicon substrate is porous silicon.

5. The method of claim 1, wherein said unactivated functionality comprises a carboxyl radical.

6. The method of claim 5, wherein carboxyl radical is activated by reaction with N-hydroxysuccinimide.