Compositions and methods for capturing, isolating, detecting, analyzing and quantifying macromolecules

• US 6,680,210 B2
• Filed: 06/28/2001
• Issued: 01/20/2004
• Est. Priority Date: 02/18/2000
• Status: Expired due to Fees

• Current Assignee: Aspira Biosystems, Inc. (Nanomune Technologies, Inc.)
• Original Assignee: Aspira Biosystems, Inc. (Nanomune Technologies, Inc.)
• Inventors: Huang, Chin-Shiou
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US09/895,643
• Publication Number:
  US 20020045274A1
• Time in Patent Office: 936 Days
• Field of Search: 427/2.13, 435/4, 436/518, 436/524, 436/531, 436/535, 436/809
• US Class Current: 436/518
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template
• CPC Class Codes: G01N 33/544 the carrier being organic
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10S 977/927 Diagnostic contrast agent

Abstract

The present invention provides imprint compositions useful for capturing, isolating, detecting and/or quantifying macromolecules in a sample, methods of making and using the same. Generally, the imprint compositions comprise a matrix material defining an imprint of a template molecule, and the template molecule typically corresponds to a portion of a macromolecule of interest.

Claims

1. A method of making a spatially-identifiable array of imprint cavitites, comprising the steps of:

(a) solidifying a fluidic matrix material capable of retaining shaped cavities when a solid or semi-solid form in the presence of a spatially-identifiable array of immobilized template molecules, thereby yielding a solid or semi-solid matrix material having template molecules entrapped therein; and

(b) removing the template molecules from the solid for semi-solid matrix material, yielding a spatially-identifiable array of imprint cavities.

2. The method of claim 1 in which at least one immobilized template molecule corresponds to a portion of a macromolecule of interest.

3. The method of claim 1 in which each template molecule of the array of immobilized template molecules is unique.

4. The method of claim 1 in which each immobilized template molecule corresponds to a portion of a macromolecule of interest.

5. The method of claim 1 in which each immobilized template molecule corresponds to a portion of a different macromolecule of interest.