Nucleic acid-based detection
First Claim
1. An aptamer beacon that binds to a specific non-nucleic acid target molecule, the aptamer beacon comprisingan oligonucleotide comprising a loop portion, a first segment, and a second segment complementary to the first segment, wherein the first and second segments connected by the loop portion form a stem portion when hybridized together;
- wherein a portion of the oligonucleotide comprises a binding region that has a secondary or tertiary conformation that changes to a different secondary or tertiary conformation upon specifically binding to the non-nucleic acid target molecule;
a first reporter moiety attached to the first segment; and
a second reporter moiety attached to the second segment, wherein the first and second reporter moieties interact to produce a detectable signal when the distance between them is changed;
wherein specific binding of the target molecule to the binding region causes a change in conformation of the aptamer beacon that separates the first and second segments, thereby altering the distance between the first and second reporter moieties, and produces a detectable signal.
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Accused Products
Abstract
The invention relates to compositions, systems, and methods for simultaneously detecting the presence and quantity of one or more different compounds in a sample using aptamer beacons. Aptamer beacons are oligonucleotides that have a binding region that can bind to a non-nucleotide target molecule, such as a protein, a steroid, or an inorganic molecule. New aptamer beacons having binding regions configured to bind to different target molecules can be used in solution-based and solid, array-based systems. The aptamer beacons can be attached to solid supports, e.g., at different predetermined points in two-dimensional arrays. The invention includes devices, methods, and computer software for carrying out the methods.
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Citations
13 Claims
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1. An aptamer beacon that binds to a specific non-nucleic acid target molecule, the aptamer beacon comprising
an oligonucleotide comprising a loop portion, a first segment, and a second segment complementary to the first segment, wherein the first and second segments connected by the loop portion form a stem portion when hybridized together; -
wherein a portion of the oligonucleotide comprises a binding region that has a secondary or tertiary conformation that changes to a different secondary or tertiary conformation upon specifically binding to the non-nucleic acid target molecule;
a first reporter moiety attached to the first segment; and
a second reporter moiety attached to the second segment, wherein the first and second reporter moieties interact to produce a detectable signal when the distance between them is changed;
wherein specific binding of the target molecule to the binding region causes a change in conformation of the aptamer beacon that separates the first and second segments, thereby altering the distance between the first and second reporter moieties, and produces a detectable signal. - View Dependent Claims (2, 3, 4, 5, 6, 10, 11)
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7. An aptamer beacon that binds to a non-nucleic acid target molecule, the aptamer beacon comprising
an oligonucleotide comprising a first segment, a second segment, and a third segment, wherein the first and second segments can form base pair bonds to form a complex when the aptamer beacon is not bound to the target molecule; -
wherein a portion of the oligonucleotide comprises a binding region having a secondary or tertiary conformation that changes to a different secondary or tertiary conformation upon binding to the non-nucleic acid target molecule;
a first reporter moiety attached to the first segment; and
a second reporter moiety attached to the second segment, wherein the first and second reporter moieties interact to produce a detectable signal when the distance between them is changed;
wherein binding of the target molecule to the binding region causes a change in conformation of the aptamer beacon that alters the distance between the first end second reporter moieties, and produces a detectable signal. - View Dependent Claims (8, 9)
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12. An aptamer beacon that binds to a non-nucleic acid target molecule, the aptamer beacon comprising
an oligonucleotide comprising a first segment, a second segment, and a third segment, wherein the first and second segments can form base pair bonds to form a complex when the aptamer beacon is not bound to the target molecule; -
wherein a portion of the oligonucleotide comprises a binding region having a secondary or tertiary conformation that changes to a different secondary or tertiary conformation upon binding to the non-nucleic acid target molecule;
a first reporter moiety attached to the first segment; and
a second reporter moiety attached to the third segment, wherein the first and second reporter moieties interact to produce a detectable signal when the distance between them is changed;
wherein binding of the target molecule to the binding region causes a change in conformation of the aptamer beacon that alters the distance between the first and second reporter moieties, and producing a detectable signal. - View Dependent Claims (13)
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Specification