Amplification and detection of organisms of the Chlamydiaceae family
First Claim
Patent Images
1. An oligonucleotide consisting of a target binding sequence selected from the group consisting of the target binding sequences of CGLP 1.0 (SEQ ID NO:
- 1), Rnp LP (SEQ ID NO;
2), CGRP1.0 (SEQ ID NO;
3) and Rnp RP (SEQ ID NO;
4), and optionally, a sequence required for an amplification reaction.
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Abstract
Amplification primers and methods for specific amplification and detection of a rnpB gene sequence are disclosed. The primer-target binding sequences are useful for amplification and detection of organisms of the Chlamydiaceae family in a variety of amplification and detection reactions.
145 Citations
31 Claims
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1. An oligonucleotide consisting of a target binding sequence selected from the group consisting of the target binding sequences of CGLP 1.0 (SEQ ID NO:
- 1), Rnp LP (SEQ ID NO;
2), CGRP1.0 (SEQ ID NO;
3) and Rnp RP (SEQ ID NO;
4), and optionally, a sequence required for an amplification reaction. - View Dependent Claims (2, 3)
- 1), Rnp LP (SEQ ID NO;
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4. An oligonucleotide selected from the group consisting of CGLB1.0 (SEQ ID NO:
- 5), Rnp LB (SEQ ID NO;
6), CGRB1.0 (SEQ ID NO;
7) and Rnp RB (SEQ ID NO;
8).
- 5), Rnp LB (SEQ ID NO;
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5. An oligonucleotide selected from the group consisting of CGA1.0 (SEQ ID NO:
- 9), a nucleic acid complementary to CGA1.0 (SEQ ID NO;
9), Rnp AD (SEQ ID NO;
10) and a nucleic acid complementary to Rnp AD (SEQ ID NO;
10). - View Dependent Claims (6, 7)
- 9), a nucleic acid complementary to CGA1.0 (SEQ ID NO;
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8. A pair of amplification primers comprising:
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a) a first primer consisting of a target binding sequence selected from the group consisting of the target binding sequences of CGLP1.0 (SEQ ID NO;
1) and Rnp LP (SEQ ID NO;
2), and, optionally, a sequence required for an amplification reaction, and;
b) a second primer consisting of a target binding sequence selected from the group consisting of the target binding sequences of CGRP1.0 (SEQ ID NO;
3) and Rnp RP (SEQ ID NO;
4), and, optionally, a sequence required for an amplification reaction.- View Dependent Claims (9, 10, 11)
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12. A kit comprising:
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a) one or more primers selected from the group consisting of CGLP1.0 (SEQ ID NO;
1) and Rnp LP (SEQ ID NO;
2),b) one or more primers selected from the group consisting of CGRP1.0 (SEQ ID NO;
3) and Rnp RP (SEQ ID NO;
4),c) one or more bumpers selected from the group consisting of CGLB1.0 (SEQ ID NO;
5), Rnp LB (SEQ ID NO;
6), CGRB1.0 (SEQ ID NO;
7) and Rnp RB (SEQ ID NO;
8), andd) one or more signal primers selected from the group consisting of CGA1.0 (SEQ ID NO;
9), a nucleic acid complementary to CGA1.0 (SEQ ID NO;
9), Rnp AD (SEQ ID NO;
10) and a nucleic acid complementary to Rnp AD (SEQ ID NO;
10).- View Dependent Claims (13, 14, 15, 16)
e) a pair of primers specific for the amplification of a nucleic acid sequence specific for Bordetella pertussis;
f) a pair of primers specific for the amplification of a nucleic acid sequence specific for Mycoplasma pneumoniae; and
g) a pair of primers specific for the amplification of a nucleic acid sequence specific for Legionella pneumophila.
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17. A method for detecting the presence or absence of organisms of the Chlamydiaceae family in a sample, said method comprising:
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a) treating said sample using a pair of nucleic acid primers in a nucleic acid amplification reaction wherein a first primer is selected from the group consisting of CGLP1.0 (SEQ ID NO;
1) and Rnp LP (SEQ ID NO;
2), and said second primer is selected from the group consisting of CGRP1.0 (SEQ ID NO;
3) and Rnp RP (SEQ ID NO;
4), andb) detecting any amplified nucleic acid product, wherein detection of amplified product indicates presence of organisms of the Chlamydiaceae family. - View Dependent Claims (18, 19, 20, 21, 22)
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23. A method for amplifying a target nucleic acid sequence from an organism of the Chlamydiaceae family comprising:
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a) hybridizing to the nucleic acid i) a first amplification primer selected from the group consisting of a target binding sequence of CGLP1.0 (SEQ ID NO;
1) and a target binding sequence of Rnp LP (SEQ ID NO;
2), and, optionally, a sequence required for an amplification reaction, andii) a second amplification primer selected from the group consisting of a target binding sequence of CGRP1.0 (SEQ ID NO;
3) and a target binding sequence of Rnp RP (SEQ ID NO;
4), and, optionally, a sequence required for the amplification reaction, and;
b) extending the hybridized first and second amplification primers on the target nucleic acid sequence whereby the target nucleic acid sequence is amplified. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31)
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Specification