Methods of diagnosing and determining prognosis of colorectal cancer

• US 6,682,890 B2
• Filed: 05/08/2001
• Issued: 01/27/2004
• Est. Priority Date: 08/17/2000
• Status: Expired due to Fees

• Current Assignee: Facet Biotech Corporation
• Original Assignee: Protein Design Labs, Inc. (PDL BioPharma, Inc.)
• Inventors: Mack, David, Gish, Kurt C., Wilson, Keith E.
• Primary Examiner(s): Horlick, Kenneth R.
• Assistant Examiner(s): KIM, YOUNG J
• Application Number:
  US09/851,588
• Publication Number:
  US 20020042067A1
• Time in Patent Office: 994 Days
• Field of Search: 435/6, 435/91.1, 435/91.2, 336/23.1, 530/300, 530/387.1
• US Class Current: 435/6.14
• CPC Class Codes: A61K 49/0008 Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/118 Prognosis of disease development
• CPC Class Codes: C12Q 2600/136 Screening for pharmacological compounds
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: G01N 33/5011 for testing antineoplastic activity
• CPC Class Codes: G01N 33/57419 of colon

Abstract

Described herein are methods that can be used for diagnosis and prognosis of colorectal cancer. Also described herein are methods that can be used to screen candidate bioactive agents for the ability to modulate colorectal cancer. Additionally, methods and molecular targets (genes and their products) for therapeutic intervention in colorectal cancer are described.

Claims

1. A method of detecting colorectal cancer comprising:

a) determining the expression of a gene encoding CGA7 in a first colon tissue of a first individual; and

b) comparing said expression of said gene with that of a normal colon tissue obtained from said first individual or a second unaffected individual;

wherein a difference in said expression is an indication that the first individual may have colorectal cancer.

2. A method for determining the prognosis of an individual with colorectal cancer, comprising:

a) determining the expression of a gene encoding CGA7 in colorectal cancer tissues in different states of the individual; and

b) comparing the expression profile of the gene in the different states, wherein a higher level of the expression in a later state compared with an early state indicates a poor prognosis.

3. A method of detecting colorectal cancer comprising:

a) determining the amount of mRNA encoding CJA8 or a fragment thereof in a first colon tissue of a first individual; and

b) comparing said amount with that of a normal colon tissue obtained from said first individual or a second unaffected individual;

wherein a difference in said expression is an indication that the first individual may have colorectal cancer.

4. A method for determining the prognosis of an individual with colorectal cancer, comprising:

a) determining the expression of a gene encoding CJA8 in colorectal cancer tissues in different states of the individual; and

b) comparing the expression profile of the gene in the different states, wherein a higher level of the expression in a later state compared with an early state indicates a poor prognosis.