Production of γ linolenic acid by a Δ6-desaturase
First Claim
1. A method of inducing production of gamma linolenic acid (GLA) in plants, plant cells or microorganisms which are deficient or lacking in or producing low levels of GLA and linoleic acid (LA), wherein said method comprises transforming said plants, plant cells or microorganisms with an isolated nucleic acid encoding bacterial Δ
- 6-desaturase and an isolated nucleic acid encoding Δ
12-desaturase.
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Abstract
Linoleic acid is converted into γ-linolenic acid by the enzyme Δ6-desaturase. The present invention is directed to isolated nucleic acids comprising the Δ6-desaturase gene. More particularly, the isolated nucleic acid comprises the promoter, coding region and termination regions of the Δ6-desaturase gene. The present invention provides recombinant constructions comprising the Δ6-desaturase coding region in functional combination with heterologous regulatory sequences. The nucleic acids and recombinant constructions of the instant invention are useful in the production of GLA in transgenic organisms.
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Citations
10 Claims
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1. A method of inducing production of gamma linolenic acid (GLA) in plants, plant cells or microorganisms which are deficient or lacking in or producing low levels of GLA and linoleic acid (LA), wherein said method comprises transforming said plants, plant cells or microorganisms with an isolated nucleic acid encoding bacterial Δ
- 6-desaturase and an isolated nucleic acid encoding Δ
12-desaturase. - View Dependent Claims (3, 5, 6, 9)
- 6-desaturase and an isolated nucleic acid encoding Δ
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2. A method of inducing production of gamma linolenic acid (GLA) in plants, plant cells or microorganisms which are deficient or lacking in or producing low levels of GLA and linoleic acid (LA), wherein said method comprises transforming said plants, plant cells or microorganisms with at least one expression vector comprising an isolated nucleic acid encoding evening primrose Δ
- 6-desaturase and an isolated nucleic acid encoding Δ
12-desaturase. - View Dependent Claims (4, 7, 8, 10)
- 6-desaturase and an isolated nucleic acid encoding Δ
Specification