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Oligonucleotides and methods for detecting Borrelia burgdorferi

  • US 6,686,162 B2
  • Filed: 12/04/2001
  • Issued: 02/03/2004
  • Est. Priority Date: 12/04/2001
  • Status: Active Grant
First Claim
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1. A method for producing an oligonucleotide that is a hybrid of human placental DNA with FlaA nucleic acid sequence, comprising:

  • amplifying human placental DNA using a pair of oligonucleotide primers having the sequences set forth in SEQ ID NO;

    4 and SEQ ID NO;

    5, wherein said primers each further contain a terminal FlaA nucleic acid sequence comprising 5 or more contiguous nucleotides selected from 5′

    -TTG CAA ATC TTT TCT CTG GTG-3′

    (SEQ ID NO;

    1) or 5′

    -AGA ATT AAC TCC GCC TTG AGA-3′

    (SEQ ID NO;

    2) to provide a plurality of human placental-FlaA nucleic acid hybrid amplicons; and

    purifyng the human placental-FlaA nucleic acid hybrid amplicons.

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