Oligonucleotides and methods for detecting Borrelia burgdorferi
First Claim
1. A method for producing an oligonucleotide that is a hybrid of human placental DNA with FlaA nucleic acid sequence, comprising:
- amplifying human placental DNA using a pair of oligonucleotide primers having the sequences set forth in SEQ ID NO;
4 and SEQ ID NO;
5, wherein said primers each further contain a terminal FlaA nucleic acid sequence comprising 5 or more contiguous nucleotides selected from 5′
-TTG CAA ATC TTT TCT CTG GTG-3′
(SEQ ID NO;
1) or 5′
-AGA ATT AAC TCC GCC TTG AGA-3′
(SEQ ID NO;
2) to provide a plurality of human placental-FlaA nucleic acid hybrid amplicons; and
purifyng the human placental-FlaA nucleic acid hybrid amplicons.
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Accused Products
Abstract
The present invention provides methods and compositions for determining the presence and/or amount of Borrelia burgdorferi nucleic acids in a test sample related to Lyme disease. In particular, substantially purified oligonucleotide primers and probes are described that can be used for qualitatively and quantitatively detecting Borrelia burgdorferi nucleic acid in a test sample by amplification methods. The present invention also provides primers and probes for generating and detecting control nucleic acid sequences that provide a convenient method for assessing internal quality control of the Borrelia burgdorferi assay.
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Citations
1 Claim
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1. A method for producing an oligonucleotide that is a hybrid of human placental DNA with FlaA nucleic acid sequence, comprising:
-
amplifying human placental DNA using a pair of oligonucleotide primers having the sequences set forth in SEQ ID NO;
4 and SEQ ID NO;
5, wherein said primers each further contain a terminal FlaA nucleic acid sequence comprising 5 or more contiguous nucleotides selected from 5′
-TTG CAA ATC TTT TCT CTG GTG-3′
(SEQ ID NO;
1) or 5′
-AGA ATT AAC TCC GCC TTG AGA-3′
(SEQ ID NO;
2) to provide a plurality of human placental-FlaA nucleic acid hybrid amplicons; and
purifyng the human placental-FlaA nucleic acid hybrid amplicons.
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Specification
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- Resources
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Current AssigneeQuest Diagnostics Investments Incorporated (Quest Diagnostics Incorporated)
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Original AssigneeQuest Diagnostics Investments Incorporated (Quest Diagnostics Incorporated)
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InventorsExner, Maurice, Hamdan, Hasnah, Lewinski, Michael
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Primary Examiner(s)Fredman, Jeffrey
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Assistant Examiner(s)Strzelecka, Teresa
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Application NumberUS10/011,340Publication NumberTime in Patent Office791 DaysField of Search536/23.1, 536/24.3, 435/91.2, 435/6US Class Current435/6.16CPC Class CodesC12Q 1/689 for bacteriaC12Q 2600/166 Oligonucleotides used as in...Y02A 50/30 Against vector-borne diseas...
