Polynucleotide encoding a human myosin-like polypeptide expressed predominantly in heart and muscle

• US 6,686,188 B2
• Filed: 05/25/2001
• Issued: 02/03/2004
• Est. Priority Date: 05/26/2000
• Status: Expired due to Fees

• Current Assignee: GE Healthcare Limited (General Electric Company)
• Original Assignee: Amersham PLC (General Electric Company)
• Inventors: Shannon, Mark E., Chen, Wensheng, Ji, Yonggang, Hanzel, David Kagen, Rank, David Russell, Penn, Sharron Gaynor, Gu, Yizhong
• Primary Examiner(s): Prouty, Rebecca A.
• Assistant Examiner(s): Steadman, David J
• Application Number:
  US09/866,108
• Publication Number:
  US 20020048800A1
• Time in Patent Office: 984 Days
• Field of Search: 536/23.1, 536/23.2, 536/23.5, 536/24.31, 435/320.1, 435/196, 435/254.11, 435/325, 435/252.3, 435/287.2, 435/419, 514/44
• US Class Current: 506/16
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2217/075 inducing loss of function, i.e. knock out
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/4716 Muscle proteins, e.g. myosin, actin
• CPC Class Codes: C07K 14/4748 Tumour specific antigens; Tumour rejection antigen precursors [TRAP], e.g. MAGE
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C07K 2319/40 containing a tag for immunodetection, or an epitope for immunisation
• CPC Class Codes: C07K 2319/60 containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
• CPC Class Codes: C12N 15/1034 Isolating an individual clone by screening libraries
• CPC Class Codes: C12N 15/66 General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/136 Screening for pharmacological compounds
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: G16B 25/00 ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
• CPC Class Codes: G16B 25/10 : Gene or protein expression profiling; Expression-ratio estimation or normalisation
• CPC Class Codes: G16B 25/20 : Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
• CPC Class Codes: G16B 30/00 : ICT specially adapted for sequence analysis involving nucleotides or amino acids
• CPC Class Codes: G16B 30/10 : Sequence alignment; Homology search
• CPC Class Codes: Y02A 90/10 : Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Abstract

Presented are a novel myosin-like protein particularly expressed in human heart and muscle, isolated nucleic acids encoding the myosin-like protein, compounds and compositions derivable directly or indirectly therefrom, and diagnostic and therapeutic methods for using the same.

Claims

1. An isolated nucleic acid comprising:

a polynucleotide having a sequence selected from the group consisting of (i) the nucleotide sequence of SEQ ID NO;

1, (ii) the nucleotide sequence of SEQ ID NO;

2, (iii) a nucleotide sequence that is a degenerate variant of the nucleotide sequence of SEQ ID NO;

2, (iv) a nucleotide sequence at least 90% identical in sequence to SEQ ID NO;

2, (v) a nucleotide sequence that encodes a polypeptide with the amino acid sequence of SEQ ID NO;

3, (vi) a nucleotide sequence that encodes a polypeptide at least 90% identical in sequence to SEQ ID NO;

3, and (vii) a nucleotide sequence that is the complete complement of the nucleotide sequence of any one of (i)-(vi), wherein said nucleic acid encodes a polypeptide having ATPase activity or a polypeptide capable of binding calmodulin.

4. The isolated nucleic acid of any one of claims 1-3, wherein said nucleic acid encodes a polypeptide having ATPase activity.

5. The isolated nucleic acid of any one of claims 1-3, wherein said nucleic acid encodes a polypeptide capable of binding calmodulin.

6. The isolated nucleic acid of any one of claims 1-3, wherein said nucleic acid is expressed in skeletal muscle and heart muscle.

7. The isolated nucleic acid of any one of claims 1-3, further comprising at least one expression control element, wherein said polynucleotide is operably linked to said at least one expression control element.

8. The isolated nucleic acid of any one of claims 1-3, attached to a substrate.

9. A microarray wherein at least one probe of said array is a nucleic acid according to any one of claims 1-3.

10. A replicable vector comprising the isolated nucleic acid of any one of claims 1-3.

11. A replicable vector comprising the isolated nucleic acid of claim 7.

12. An expression vector comprising the isolated nucleic acid of any one of claims 1-3.

13. A host cell transformed to contain the nucleic acid of any one of claims 1-3, or the progeny thereof containing said nucleic acid.

14. A host cell transformed to contain the replicable vector of claim 10, or the progeny thereof containing said replicable vector.

15. A host cell transformed to contain the replicable vector of claim 11, or the progeny thereof containing said replicable vector.

16. A host cell transformed to contain the expression vector of claim 12, or the progeny thereof containing said expression vector.

17. A method for producing a polypeptide, the method comprising:

culturing the host cell of claim 15 under conditions in which the protein encoded by said nucleic acid is expressed.

18. The method of claim 17, further comprising the step of:

isolating said expressed protein.

19. A method for producing a polypeptide, the method comprising:

culturing the host cell of claim 16 under conditions in which the protein encoded by said nucleic acid is expressed.

20. The method of claim 19, further comprising the step of:

isolating said expressed protein.

21. The isolated nucleic acid of claim 4, wherein said nucleic acid is expressed in skeletal muscle and heart muscle.

22. The isolated nucleic acid of claim 5, wherein said nucleic acid is expressed in skeletal muscle and heart muscle.

23. The isolated nucleic acid of claim 4, further comprising at least one expression control element, wherein said polynucleotide is operably linked to said at least one expression control element.

24. The isolated nucleic acid of claim 5, further comprising at least one expression control element, wherein said polynucleotide is operably linked to said at least one expression control element.

25. The isolated nucleic acid of claim 4, attached to a substrate.

26. The isolated nucleic acid of claim 5, attached to a substrate.

27. A microarray wherein at least one probe of said array is a nucleic acid according to claim 4.

28. A microarray wherein at least one probe of said array is a nucleic acid according to claim 5.

29. A replicable vector comprising the isolated nucleic acid of claim 4.

30. A replicable vector comprising the isolated nucleic acid of claim 5.

31. A replicable vector comprising the isolated nucleic acid of claim 23.

32. A replicable vector comprising the isolated nucleic acid of claim 24.

33. An expression vector comprising the isolated nucleic acid of claim 4.

34. An expression vector comprising the isolated nucleic acid of claim 5.

35. A host cell transformed to contain the nucleic acid of claim 4, or the progeny thereof containing said nucleic acid.

36. A host cell transformed to contain the nucleic acid of claim 5, or the progeny thereof containing said nucleic acid.

37. A host cell transformed to contain the replicable vector of claim 29, or the progeny thereof containing said replicable vector.

38. A host cell transformed to contain the replicable vector of claim 30, or the progeny thereof containing said replicable vector.

39. A host cell transformed to contain the replicable vector of claim 31, or the progeny thereof containing said replicable vector.

40. A host cell transformed to contain the replicable vector of claim 32, or the progeny thereof containing said replicable vector.

41. A host cell transformed to contain the expression vector of claim 33, or the progeny thereof containing said expression vector.

42. A host cell transformed to contain the expression vector of claim 34, or the progeny thereof containing said expression vector.

43. A method for producing a polypeptide, the method comprising:

culturing the host cell of claim 39 under conditions in which the protein encoded by said nucleic acid is expressed.

44. A method for producing a polypeptide, the method comprising:

culturing the host cell of claim 40 under conditions in which the protein encoded by said nucleic acid is expressed.

45. The method of claim 43, further comprising the step of:

isolating said expressed protein.

46. The method of claim 44, further comprising the step of:

isolating said expressed protein.

47. A method for producing a polypeptide, the method comprising:

culturing the host cell of claim 41 under conditions in which the protein encoded by said nucleic acid is expressed.

48. A method for producing a polypeptide, the method comprising:

culturing the host cell of claim 42 under conditions in which the protein encoded by said nucleic acid is expressed.

49. The method of claim 47, further comprising the step off:

isolating said expressed protein.

50. The method of claim 48, further comprising the step of:

isolating said expressed protein.

2. An isolated nucleic acid comprising:

a polynucleotide having a sequence selected from the group consisting of (i) the nucleotide sequence of SEQ ID NO;

1, (ii) the nucleotide sequence of SEQ ID NO;

2, (iii) a nucleotide sequence that is a degenerate variant of the nucleotide sequence of SEQ ID NO;

2, (iv) a nucleotide sequence at least 95% identical in sequence to SEQ ID NO;

2, (v) a nucleotide sequence that encodes a polypeptide with the amino acid sequence of SEQ ID NO;

3, (vi) a nucleotide sequence that encodes a polypeptide at least 95% identical in sequence to SEQ ID NO;

3, and (vii) a nucleotide sequence that is the complete complement of the nucleotide sequence of any one of (i)-(vi), wherein said nucleic acid encodes a polypeptide having ATPase activity or a polypeptide capable of binding calmodulin.

3. An isolated nucleic acid comprising:

a polynucleotide having a sequence selected from the group consisting of (i) the nucleotide sequence of SEQ ID NO;

1, (ii) the nucleotide sequence of SEQ ID NO;

2, (iii) a nucleotide sequence that is a degenerate variant of the nucleotide sequence of SEQ ID NO;

2, (iv) a nucleotide sequence at least 99% identical in sequence to SEQ ID NO;

2, (v) a nucleotide sequence that encodes a polypeptide with the amino acid sequence of SEQ ID NO;

3, (vi) a nucleotide sequence that encodes a polypeptide at least 99% identical in sequence to SEQ ID NO;

3, and (vii) a nucleotide sequence that is the complete complement of the nucleotide sequence of any one of (i)-(vi), wherein said nucleic acid encodes a polypeptide having ATPase activity or a polypeptide capable of binding calmodulin.