Optical system and method for optically analyzing light from a sample
First Claim
1. An optical system for analyzing light from a plurality of samples, comprising:
- a plurality of sample holders;
a collection lens configured to receive and substantially collimate light from the plurality of sample holders;
a substantially hemispherical optical element comprising a flat surface facing the plurality of sample holders, a hemispherically curved surface facing said collection lens, and a radius of curvature, the center of said radius of curvature being located substantially on an optical axis of the collection lens and substantially at or near an object plane of the collection lens;
a transmission grating configured to spectrally disperse the substantially collimated light from the collection lens; and
a reimaging lens configured to receive the spectrally dispersed light from the transmission grating and direct the spectrally dispersed light onto a light detection device.
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Accused Products
Abstract
An optical system for analyzing light from a plurality of samples is provided. The optical system includes a plurality of holders adapted to have samples located therein, a collection lens, a transmission grating, and a reimaging lens. The collection lens is configured to receive and substantially collimate light from the samples. The transmission grating is configured to spectrally disperse the substantially collimated light from the collection lens. The reimaging lens is configured to receive the light from the light dispersing element and direct the light onto a light detection device. A method of optically analyzing at least one sample is also provided.
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Citations
191 Claims
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1. An optical system for analyzing light from a plurality of samples, comprising:
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a plurality of sample holders;
a collection lens configured to receive and substantially collimate light from the plurality of sample holders;
a substantially hemispherical optical element comprising a flat surface facing the plurality of sample holders, a hemispherically curved surface facing said collection lens, and a radius of curvature, the center of said radius of curvature being located substantially on an optical axis of the collection lens and substantially at or near an object plane of the collection lens;
a transmission grating configured to spectrally disperse the substantially collimated light from the collection lens; and
a reimaging lens configured to receive the spectrally dispersed light from the transmission grating and direct the spectrally dispersed light onto a light detection device. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. An optical system for analyzing light from a plurality of samples, comprising:
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a plurality of sample holders;
a collection lens configured to receive and substantially collimate light from the plurality of sample holders;
a substantially hemispherical optical element comprising a flat surface facing the plurality of sample holders, a hemispherically curved surface facing said collection lens, and a radius of curvature, the center of said radius of curvature being located substantially on an optical axis of the collection lens and substantially at or near an object plane of the collection lens;
a transmission grating configured to spectrally disperse the substantially collimated light from the collection lens; and
a reimaging lens configured to receive the spectrally dispersed light from the transmission grating and direct the light onto a light detection device, wherein said system is configured to compensate for chromatic aberration in the spectrally dispersed light, the compensation comprising an optical axis of the reimaging lens being non-perpendicular with a surface of the light detection device. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64)
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65. An optical system for analyzing light from a plurality of samples, comprising:
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a plurality of sample holders;
a collection lens configured to receive and substantially collimate light from the plurality of sample holders;
a transmission grating configured to spectrally disperse the substantially collimated light from the collection lens; and
a reimaging lens configured to receive the spectrally dispersed light from the transmission grating and direct the light onto a light detection device, wherein said system is configured to compensate for chromatic aberration in the spectrally dispersed light, the compensation comprising an optical axis of the reimaging lens being non-perpendicular with a surface of the light detection device. - View Dependent Claims (66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90)
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91. A system for analyzing light from a sample in a separation system, comprising:
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at least one separation lane;
a collection lens configured to receive and substantially collimate light emitted from the at least one separation lane;
a substantially hemispherical optical element comprising a flat surface facing the at least one separation lane, a hemispherically curved surface facing said collection lens, and a radius of curvature, the center of said radius of curvature being located substantially on an optical axis of the collection lens and substantially at or near an object plane of the collection lens;
a light dispersing element configured to spectrally disperse substantially collimated light from the collection lens; and
a reimaging lens configured to receive light from the light dispersing element and direct the light onto a light detection device, wherein said system is configured to compensate for chromatic aberration in the spectrally dispersed light, the compensation comprising an optical axis of the reimaging lens being non-perpendicular with a surface of the light detection device. - View Dependent Claims (92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107)
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108. A system for analyzing light from a light source, comprising:
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a collection lens configured to receive and substantially collimate light emitted from the light source;
a substantially hemispherical optical element comprising a flat surface facing the light source, a hemispherically curved surface facing said collection lens, and a radius of curvature, the center of said radius of curvature being located substantially on an optical axis of the collection lens and substantially at or near an object plane of the collection lens;
a light dispersing element configured to spectrally disperse the substantially collimated light from the collection lens; and
a reimaging lens configured to receive the spectrally dispersed light from the light dispersing element and direct the light onto a light detection device, wherein said system is configured to compensate for chromatic aberration in the spectrally dispersed light, the compensation comprising an optical axis of the reimaging lens being non-perpendicular with a surface of the light detection device. - View Dependent Claims (109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125)
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126. A system for analyzing light from a sample in a separation system, comprising:
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at least one separation lane;
a collection lens configured to receive and substantially collimate light emitted from the at least one separation lane;
a substantially hemispherical optical element comprising a flat surface facing the at least one separation lane, a hemispherically curved surface facing said collection lens, and a radius of curvature, the center of said radius of curvature being located substantially on an optical axis of the collection lens and substantially at or near an object plane of the collection lens;
a transmission grating configured to spectrally disperse the substantially collimated light from the collection lens; and
a reimaging lens configured to receive the spectrally dispersed light from the transmission grating and direct the spectrally dispersed light onto a light detection device, wherein said system is configured to compensate for chromatic aberration in the spectrally dispersed light, the compensation comprising an optical axis of the reimaging lens being non-perpendicular with a surface of the light detection device. - View Dependent Claims (127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140)
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141. A method of optically analyzing a plurality of samples, comprising:
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providing a plurality of samples;
illuminating the samples with an excitation light to generate an emission light;
collecting the emission light emitted from the samples with a collection lens, said collecting comprising collecting the emission light through a substantially hemispherical element located between the collection lens and the plurality of samples, wherein the substantially hemispherical optical element comprises a flat surface facing the plurality of samples, a hemispherically curved surface facing said collection lens, and a radius of curvature, the center of said radius of curvature being located substantially on an optical axis of the collection lens and substantially at or near an object plane of the collection lens;
substantially collimating the emission light with the collection lens;
spectrally dispersing the substantially collimated emission light with a transmission grating;
directing the spectrally dispersed emission light from the transmission grating onto a light detection device by a reimaging lens; and
optically detecting the spectral characteristics of the spectrally dispersed emission light with a light detection device, wherein said excitation light does not pass through the collection lens prior to illuminating the sample; and
further comprising compensating for chromatic aberration in the spectrally dispersed emission light, the compensating comprising configuring an optical axis of the reimaging lens to be non-perpendicular with a surface of the light detection device. - View Dependent Claims (142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154)
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155. A method of optically analyzing a plurality of samples, comprising:
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providing a plurality of samples;
illuminating the plurality of samples with an excitation light to generate an emission light;
collecting the emission light emitted from the plurality of samples with a collection lens, said collecting comprising collecting the emission light through a substantially hemispherical element located between the collection lens and the plurality of samples, wherein the substantially hemispherical optical element comprises a flat surface facing the plurality of samples, a hemispherically curved surface facing said collection lens, and a radius of curvature, the center of said radius of curvature being located substantially on an optical axis of the collection lens and substantially at or near an object plane of the collection lens;
substantially collimating the emission light with the collection lens;
spectrally dispersing the substantially collimated emission light with a transmission grating;
directing the spectrally dispersed emission light from the transmission grating onto a light detection device by a reimaging lens; and
optically detecting spectral characteristics of the spectrally dispersed emission light, wherein said excitation light does not pass through the collection lens prior to illuminating the sample. - View Dependent Claims (156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169)
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170. A method of optically analyzing a plurality of samples, comprising:
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providing a plurality of samples;
illuminating the plurality of samples with an excitation light to generate an emission light;
collecting the emission light emitted from the sample with a collection lens;
substantially collimating the emission light with the collection lens;
spectrally dispersing the substantially collimated emission light with a transmission grating;
directing the spectrally dispersed emission light from the transmission grating onto a light detection device by a reimaging lens; and
optically detecting spectral characteristics of the spectrally dispersed emission light, wherein said excitation light does not pass through the collection lens prior to illuminating the sample; and
further comprising compensating for chromatic aberration in the spectrally dispersed emission light, the compensating comprising configuring an optical axis of the reimaging lens to be non-perpendicular with a surface of the light detection device. - View Dependent Claims (171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183)
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184. An optical system for analyzing light from a plurality of samples, comprising:
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a plurality of sample holders;
a collection lens configured to receive and substantially collimate light from the plurality of sample holders;
an optical element comprising at least a hemispherical portion having a diametral plane and a curved surface facing the collection lens, a flat surface facing the plurality of sample holders, and a radius of curvature, the center of said radius of curvature being located substantially on an optical axis of the collection lens and substantially at or near an object plane of the collection lens;
a transmission grating configured to spectrally disperse the substantially collimated light from the collection lens; and
a reimaging lens configured to receive the spectrally dispersed light from the transmission grating and direct the spectrally dispersed light onto a light detection device. - View Dependent Claims (185, 186, 187, 189, 190, 191)
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188. The system of claim 256, wherein the more than one width comprises widths that decrease along the extension in the direction away from the curved surface.
Specification