Heat-reduction methods and systems related to microfluidic devices

• US 6,692,700 B2
• Filed: 02/14/2001
• Issued: 02/17/2004
• Est. Priority Date: 02/14/2001
• Status: Expired due to Term

• Current Assignee: HandyLab Incorporated (Becton, Dickinson & Co)
• Original Assignee: HandyLab Incorporated (Becton, Dickinson & Co)
• Inventors: Handique, Kalyan
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Handy, Dwayne K
• Application Number:
  US09/783,225
• Publication Number:
  US 20020110492A1
• Time in Patent Office: 1,098 Days
• Field of Search: 204/600, 204/601, 204/451, 204/424, 204/425, 204/426, 204/400, 204/193, 422/68.1, 422/82.01, 422/99, 422/100, 422/102, 436/174, 436/147, 436/180
• US Class Current: 422/503
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1883 using thermal insulation
• CPC Class Codes: B01L 2300/1894 Cooling means; Cryo cooling
• CPC Class Codes: B01L 2400/0442 thermal energy, e.g. vaporisation, bubble jet
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: H05B 3/22 non-flexible
• CPC Class Codes: H05K 3/10 in which conductive material is applied to the insulating support in such a manner as to form the desired conductive pattern
• CPC Class Codes: Y10T 29/49155 Manufacturing circuit on or in base

Abstract

A system and method for preventing or reducing unwanted heat in a microfluidic of the device while generating heat in selected regions of the device.

In one example, current is supplied to a heating element through electric leads, wherein the leads are designed so that the current density in the leads is substantially lower than the current density in the heating element. This may be accomplished using conductive leads which have a cross-sectional area which is substantially greater than the cross-sectional area of the heating element.

In another example, unwanted heat in the microfluidic complex is reduced by thermally isolating the electric leads from the microfluidic complex. This may be accomplished by running each lead directly away from the microfluidic complex, through a thermally isolating substrate. After the leads pass through the thermally isolating substrate, they are then routed to the current source. Thus, the thermally isolating substrate substantially blocks the transfer of heat from the leads to the microfluidic complex.

In another example, unwanted heat is removed from selected regions of the microfluidic complex using one or more cooling devices. One or more Peltier cooling devices may be attached to a substrate to remove heat generated by heating elements and/or other electronic circuitry.

Claims

1. A microfluidic device comprising:

a first planar substrate having a first side;

a second planar substrate having a first side and an opposing second side;

a third planar substrate having a first side;

wherein (a) the respective first sides of the first and second planar substrates abut one another and (b) the second side of the second planar substrate and the first side of the third planar substrate abut one another and further wherein the respective first sides of the first and second planar substrates comprise therebetween a microfluidic complex having a heating region, said heating region including a heating surface;

a heating element sandwiched between the first side of the second planar substrate and the second side of the second planar substrate and separated from fluid present within the heating region by a portion of the second planar substrate, the heating element configured to provide heat to said heating region through said heating surface; and

a conductive lead for supplying electric current from a current source to said heating element, said conductive lead being substantially more conductive than said heating element so as to reduce heat emitted by said conductive lead.

2. The device of claim 1 wherein said conductive lead is sufficiently thicker than said heating element to thereby render said lead substantially more conductive than said heating element.

3. A device as in claim 1, further comprising a thermally isolating layer, wherein said heating element is positioned between said heating surface and said thermally isolating layer, and said conductive lead passes from said heating element, through said thermally isolating layer, and to a current source, so as to reduce heat transfer from said conductive lead to said microfluidic complex.

4. A device for microfluidic processing, comprising:

a first substrate comprising a first side;

a second substrate comprising first and second sides, the first side of the second substrate comprising a planar oxide layer having a first side, the first side of the planar oxide layer affixed to said first substrate, the first side of the planar oxide layer and the first side of the first substrate comprising therebetween a microfluidic complex;

a heating element lying within said oxide layer, for heating a selected region of said complex, the heating element being separated by at least a portion of the oxide layer from fluid present with the selected region; and

a conductive lead for supplying electric current to said heating element, said conductive lead having a first end connected to said heating element and a second end for connection to a current source, said conductive lead being substantially thicker than said heating element so as to reduce heat emitted from said conductive lead.

5. A device as in claim 4, wherein said conductive lead has a length, and said conductive lead is substantially thicker than said heating element in a direction parallel to the plane of said planar oxide layer and orthogonal to said length.

6. A device as in claim 4, wherein said conductive lead is substantially thicker than the said heating element in a direction orthogonal to the plane of said planar oxide layer.

7. A microfluidic device comprising:

a microfluidic complex having a heating chamber which includes at least one heating surface;

a thermally isolating layer, adjacent to said heating surface, having a low thermal conductivity,

a heating element, positioned between said heating surface and said thermally isolating layer, for providing heat to said heating chamber through said heating surface; and

a conductive lead for supplying electric current from a current source to said heating element, said conductive lead passing from said heating element, through said thermally isolating layer, and to said current source, so as to substantially thermally isolate said conductive lead from said microfluidic complex.

8. A device for microfluidic processing, comprising:

a first substrate comprising a first side;

a second substrate comprising first and second opposed sides, the first side of the second substrate being affixed to said first side of the first substrate, wherein the respective first sides of the first and second substrates define a microfluidic complex therebetween;

a heating element configured to heat a selected region of said complex;

a first conductive lead extending from a first location spaced apart laterally from the heating element to a second location located closer to the heating element than the first location, the first conductive lead being separated from the first side of the first substrate by the second substrate; and

a second conductive lead connecting said heating element to said first conductive lead, wherein said second conductive lead is routed through at least the second side of said second substrate.

9. A device as in claim 8, wherein said second substrate comprises a hole extending through at least the second side thereof and the second conductive lead comprises a conductive coating on a surface of the hole.

10. A device as in claim 8, wherein said second substrate comprises a hole extending through at least the second side thereof and the second conductive lead comprises a conductive material that fills the hole.

11. A device as in claim 8, wherein said second conductive lead comprises a plurality of spaced apart conductors, the plurality of spaced apart conductors extending between the first and second sides of the second substrate.

12. A device as in claim 8, wherein said second substrate comprises a hole extending through at least the second side thereof and the second conductive lead extends through the hole.

13. A device for microfluidic processing, comprising:

a first substrate, the first substrate comprising a first side and an opposing second side;

a second substrate, the second substrate comprising a first side and an opposing second side, the first side of the second substrate affixed to said first side of the first substrate, the respective first sides of the first and second substrates comprising a microfluidic complex therebetween;

a heating element embedded within the second substrate, the heating element configured to heat, a first region of said microfluidic complex, a portion of the second substrate separating the heating element from fluid present within the first region of said complex; and

a Peltier device spaced apart from the heating element for cooling a second, different region of the complex.

14. The device of claim 13 further, comprising:

a plurality of Peltier devices affixed to said second substrate, wherein each of said plurality of Peltier devices is affixed to the side of said second substrate opposite the side of said second substrate nearest said first substrate and in a location selected to optimize cooling of a selected component of said microfluidic device.

15. A device for microfluidic processing, comprising:

a first substrate having first and second sides;

a second substrate having first and second sides, the first side of the second substrate affixed to the first side of said first substrate, the respective first sides of the first and second substrates comprising a microfluidic complex therebetween;

a heating element embedded within the second substrate and separated from the first side of said first substrate by the first side of said second substrate, for heating a selected region of said complex; and

at least one Peltier device configured to cool a selected region of the device and spaced apart from at least a portion of the microfluidic complex by at least one of the first side of said first substrate and the first side of the second substrate.

16. A device for microfluidic processing, comprising:

a first substrate comprising a first side;

a second substrate comprising first and second sides, the first side of the second substrate affixed to the first side of said first substrate the respective first sides of the first and second substrates defining a microfluidic complex;

a third substrate having first and second sides, the first side of the third substrate affixed to the second side of the second substrate;

a heating element between the first side of said first substrate and the second side of said third substrate, for heating a selected region of said complex;

a first conductive lead affixed to the second side of said third substrate;

a second conductive lead connecting said heating element to said first conductive lead, wherein said second conductive lead is routed through said third substrate; and

a Peltier device affixed to the second side of said third substrate.

17. A device of claim 16 further comprising:

a plurality of Peltier devices affixed to said third substrate.

21. The device of claim 16, wherein the second substrate is an oxide layer.

18. A device for microfluidic processing, comprising:

a first substrate comprising a first side;

a second substrate comprising first and second opposed sides, wherein the respective first sides of the first and second substrates define a microfluidic complex therebetween, the microfluidic complex comprising a cavity;

a heating element configured to heat material disposed within the cavity of the complex, the heating element spaced apart from the cavity by the first side of the second substrate;

a first conductive lead extending from a first location spaced apart laterally from the heating element to a second location located closer to the heating element than the first location, the first conductive lead being spaced apart from the first side of the first substrate by the second side of the second substrate; and

a second conductive lead connecting the heating element and the first conductive lead, wherein the second conductive lead extends through the second side of said second substrate.

19. The device of claim 18, further comprising:

a third substrate comprising first and second sides, the first side of the third substrate affixed to the second side of the second substrate, wherein the first conductive lead is spaced apart from the first side of the first substrate by the first side of the third substrate.

20. The device of claim 19, wherein the second conductive lead extends through the first side of the third substrate.

22. A microfluidic device, comprising:

a first planar substrate having a first side;

a second planar substrate having first and second sides;

wherein the respective first sides of the first and second planar substrates comprise a microfluidic network therebetween, the microfluidic network comprising a cavity, at least one surface of the cavity being defined by a portion of the first side of the second planar substrate;

a heating element embedded within the second planar substrate and configured to heat material present within the cavity, the heating element being separated from the cavity by the first side of the second planar substrate; and

at least one conductor for providing electrical current to the heating element, the conductor having a conductivity substantially greater than a conductivity of the heating element.