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Systems and methods for characterizing a biological condition or agent using precision gene expression profiles

  • US 6,692,916 B2
  • Filed: 03/29/2001
  • Issued: 02/17/2004
  • Est. Priority Date: 06/28/1999
  • Status: Expired due to Term
First Claim
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1. A method for determining a profile data set for a subject, based on a sample from the subject, the sample providing a source of RNAs, the method comprising:

  • using amplification for measuring the amount of RNA corresponding to at least four constituents from any one of Tables 1 through 7 and arriving at a measure of each constituent, where the profile data set comprises the measure of each constituent and wherein amplification is performed under measurement conditions (i) that are reproducible such that the coefficient of variation, on repeating the processes of using amplification and arriving at a measure, for the same sample and constituents, is less than approximately 3 percent and (ii) wherein efficiencies of amplification for all constituents differ by less than approximately ten percent.

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