Systems and methods for characterizing a biological condition or agent using precision gene expression profiles
First Claim
Patent Images
1. A method for determining a profile data set for a subject, based on a sample from the subject, the sample providing a source of RNAs, the method comprising:
- using amplification for measuring the amount of RNA corresponding to at least four constituents from any one of Tables 1 through 7 and arriving at a measure of each constituent, where the profile data set comprises the measure of each constituent and wherein amplification is performed under measurement conditions (i) that are reproducible such that the coefficient of variation, on repeating the processes of using amplification and arriving at a measure, for the same sample and constituents, is less than approximately 3 percent and (ii) wherein efficiencies of amplification for all constituents differ by less than approximately ten percent.
2 Assignments
0 Petitions
Accused Products
Abstract
Methods are provided for evaluating a biological condition of a subject using a calibrated profile data set derived from a data set having a plurality of members, each member being a quantitative measure of the amount of a subject'"'"'s RNA or protein as distinct constituents in a panel of constituents. The biological condition may be a naturally occurring physiological state or may be responsive to treatment of the subject with one or more agents. Calibrated profile data sets may be used as a descriptive record for an agent.
-
Citations
17 Claims
-
1. A method for determining a profile data set for a subject, based on a sample from the subject, the sample providing a source of RNAs, the method comprising:
-
using amplification for measuring the amount of RNA corresponding to at least four constituents from any one of Tables 1 through 7 and arriving at a measure of each constituent, where the profile data set comprises the measure of each constituent and wherein amplification is performed under measurement conditions (i) that are reproducible such that the coefficient of variation, on repeating the processes of using amplification and arriving at a measure, for the same sample and constituents, is less than approximately 3 percent and (ii) wherein efficiencies of amplification for all constituents differ by less than approximately ten percent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
storing the data set in a digital storage medium.
-
-
17. A method according to claim 16, wherein storing the data set includes storing it as a record in a database.
Specification
-
- Resources
Litigation Campaign Assessment
Thank you for your request. You will receive a custom alert email when the Litigation Campaign Assessment is available.
×
-
Current AssigneeLife Technologies Corporation (Thermo Fisher Scientific Incorporated)
-
Original AssigneeSource Precision Medicine Inc.
-
InventorsBankaitis-Davis, Danute M., Cheronis, John C., Tryon, Victor, Bevilacqua, Michael P.
-
Primary Examiner(s)Allen, Marianne P.
-
Application NumberUS09/821,850Publication NumberTime in Patent Office1,055 DaysField of Search435/6, 702/19, 702/20US Class Current435/6.13CPC Class CodesG01N 33/5088 of vertebratesG01N 33/6803 General methods of protein ...
