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Isolation of living cells and preparation of cell lines based on detection and quantification of preselected cellular ribonucleic acid sequences

  • US 6,692,965 B1
  • Filed: 11/22/2000
  • Issued: 02/17/2004
  • Est. Priority Date: 11/23/1999
  • Status: Expired due to Term
First Claim
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1. A method for generating a cell nine expressing at least one RNA of interest, comprising the steps of:

  • a) introducing into cells DNA encoding said at least one RNA of interest;

    b) exposing said cells to at least one molecular beacon that fluoresces upon hybridization to said at least one RNA of interest, wherein said molecular beacon comprises nucleotides complementary to the RNA of interest and nucleotides mutually complementary;

    c) isolating said cells that fluoresce; and

    d) generating a cell line expressing said at least one RNA of interest by growing said isolated cells.

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