Alteration of flowering time in plants

• US 6,693,228 B1
• Filed: 02/25/2000
• Issued: 02/17/2004
• Est. Priority Date: 02/25/1999
• Status: Expired due to Term

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Amasino, Richard Mark, Michaels, Scott Daniel, Schomburg, Fritz Michael, Scortecci, Katia, Sung, Si-Bum
• Primary Examiner(s): MEHTA, ASHWIN D
• Application Number:
  US09/513,775
• Time in Patent Office: 1,453 Days
• Field of Search: 435/69.1, 435/320.1, 435/468, 800/278, 800/290, 800/295, 800/298, 800/306, 536/23.6
• US Class Current: 800/290
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/827 Flower development or morphology, e.g. flowering promoting factor [FPF]

Abstract

The present invention provides genetic identification and use information about a family of genes, the FLOWERING LOCUS C (FLC) genes, that is part of the regulate the timing of the onset of flowering in plants. This information enables creation of transgenic plants in which the timing of the flowering of the plants has been selectively altered. Since these genes natively act to delay the time of flowering in plants, enhancing activity of the FLC protein delays the timing of flowering initiation while inhibiting the activity of FLC advances the timing of the start of flowering. A representative number of samples of the gene family are described. Members of the gene family are demonstrated to work in other plant species as well.

Claims

1. A transgenic plant comprising in its genome a transgene encoding a member of the Flowering Locus C (FLC) gene family, wherein expression of said transgene causes a delay in the onset of flowering in the transgenic plant compared to non-transgenic plants of the same species, wherein the member of the FLC gene family is selected form the group consisting of FLC1 from Arabidopsis thaliana (SEQ ID NO:

1), BrFLC1A from Brassica rapa (SEQ ID NO;

     7), and BrFLC1B from Brassica rapa (SEQ ID NO;

     9).

2. A seed of the transgenic plant of claim 1.

3. A seed for a transgenic plant, the seed comprising in its genome a transgene comprising a plant expressible promoter and a protein coding region for a plant Flowering Locus C1 (FLC1) protein, the plant FLC1 protein (i) having a MADS box domain, (ii) being at least 80% identical in amino acid sequence to the FLC1 protein from Arabidopsis, SEQ ID NO:

2, outside of the region of the MADS box domain, and (iii) effective when expressed in transgenic plants to cause a delay in the onset of flowering in the transgenic plant as compared to a non-transgenic plant of the same genetic background.

4. A plant grown from the seed of claim 3.

5. An isolated nucleotide sequence comprising the coding sequence for the FLC1 gene from Arabidopsis, SEQ ID NO:

1.

6. An isolated DNA sequence comprising a DNA sequence encoding the FLC1 protein from Arabidopsis, SEQ ID NO:

2.

7. A genetic construction comprising a plant expressible promoter operably connected to a protein coding sequence for a protein of the Flowering Locus C1 (FLC1) gene family, the plant FLC1 protein (i) having a MADS box domain, (ii) being at least 80% identical in amino acid sequence to the FLC1 (SEQ ID NO:

2) protein from Arabidopsis, and (iii) effective when expressed in transgenic plants to cause a delay in the onset of flowering in the transgenic plant as compared to a non-transgenic plant of the same genetic background.

8. A plant comprising in its genome the genetic construction of claim 7.

9. A genetic construction comprising a plant expressible promoter operably connected to a protein coding sequence for a protein of the FLC gene family, the plant FLC protein (i) having a MADS box domain, (ii) being at least 80% identical in amino acid sequence to the FLC1 (SEQ ID NO:

2) protein from Arabidopsis, and (iii) effective when expressed in transgenic plants to cause a delay in the onset of flowering in the transgenic plant as compared to a non-transgenic plant of the same genetic background, wherein the FLC protein is selected from the group consisting of FLC1 from Arabidopsis thaliana (SEQ ID NO;

1), BrFLC1A from Brassica rapa (SEQ ID NO;

7), and BrFLC1B from Brassica rapa (SEQ ID NO;

9).

10. A transgenic plant comprising a transgene encoding a member of the plant Flowering Locus C1 (FLC1) protein family, the plant FLC1 protein (i) having a MADS box domain, (ii) being at least 80% identical in amino acid sequence to the FLC1 (SEQ ID NO:

2) protein from Arabidopsis, and (iii) effective when expressed in transgenic plants to cause a delay in the onset of flowering in the transgenic plant as compared to a non-transgenic plant of the same genetic background, wherein flower initiation in the genetically transgenic plant occurs about 7 days after flower initiation in a non-transgenic plant of the same genetic background without the transgene while being grown under the same conditions.

11. A method of producing a transgenic plant with altered flowering characteristics comprising:

contacting a plant cell with a transgene comprising a plant expressible promoter and a protein coding sequence encoding a plant Flowering Locus C1 (FLC1) gene protein, the plant FLC1 protein (i) having a MADS box domain, (ii) being at least 80% identical in amino acid sequence to the FLC1 (SEQ ID NO;

2) protein from Arabidopsis, and (iii) effective when expressed in transgenic plants to cause a delay in the onset of flowering in the transgenic plant as compared to a non-transgenic plant of the same genetic background;

identifying a plant cell carrying the inserted transgene;

regenerating a transgenic plant from the plant cell, wherein the transgenic plant exhibits about 10% more leaves than a non-transgenic plant of the same genetic background without the transgene, wherein the number of leaves is determined when the transgenic plant and the non-transgenic plant are being grown under the same conditions.