Microfluidic methods, devices and systems for in situ material concentration
First Claim
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1. A method of concentrating a material, comprising:
- providing a first channel portion having at least first and second fluid regions disposed therein, the material having a first electrophoretic velocity in the first fluid region, and having a second electrophoretic velocity in the second fluid region, wherein the second electrophoretic velocity is less than the first electrophoretic velocity as a result of a different ionic make up of the first and second fluid regions, the first and second fluid regions being in contact at a first substantially static interface; and
electrophoresing the sample material through the first fluid region in the first channel portion toward the second fluid region, the sample material concentrating at the first substantially static interface.
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Abstract
Methods of concentrating materials within microfluidic channel networks by moving materials into regions in which overall velocities of the material are reduced, resulting in stacking of the material within those reduced velocity regions. These methods, devices and systems employ static fluid interfaces to generate the differential velocities, as well as counter-current flow methods, to concentrate materials within microscale channels.
167 Citations
37 Claims
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1. A method of concentrating a material, comprising:
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providing a first channel portion having at least first and second fluid regions disposed therein, the material having a first electrophoretic velocity in the first fluid region, and having a second electrophoretic velocity in the second fluid region, wherein the second electrophoretic velocity is less than the first electrophoretic velocity as a result of a different ionic make up of the first and second fluid regions, the first and second fluid regions being in contact at a first substantially static interface; and
electrophoresing the sample material through the first fluid region in the first channel portion toward the second fluid region, the sample material concentrating at the first substantially static interface.
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2. A method of concentrating a material, comprising:
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providing at least first and second channel portions, wherein the second channel portion intersects and being in fluid communication with the first channel portion, the first channel portion having at least first and second fluid regions disposed therein, the first fluid region comprising the material and having a conductivity that is lower than the second fluid region, the first and second fluid regions being in contact at a first substantially static interface; and
applying an electric field through the first and second fluid regions in the first channel portion to concentrate the material at the first substantially static interface. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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Specification