Absorption-enhanced differential extraction device

• US 6,695,147 B1
• Filed: 10/12/1999
• Issued: 02/24/2004
• Est. Priority Date: 06/14/1996
• Status: Expired due to Term

• Current Assignee: Washington University In St Louis
• Original Assignee: University of Washington
• Inventors: Yager, Paul, Brody, James P.
• Primary Examiner(s): Walsh, Donald P.
• Assistant Examiner(s): JOERGER, KAITLIN S
• Application Number:
  US09/416,072
• Time in Patent Office: 1,596 Days
• Field of Search: 209/155, 209/156, 209/157, 209/159, 209/160, 209/161, 209/208, 209/210, 210/198.1, 210/500.26, 210/634, 210/644, 210/645, 435/29
• US Class Current: 209/455
• CPC Class Codes: A61M 1/3679 by absorption A61M1/3675 takes precedence
• CPC Class Codes: B01D 15/3804 Affinity chromatography
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00828 Silicon wafers or plates
• CPC Class Codes: B01J 2219/00952 Sensing operations
• CPC Class Codes: B01J 2219/00995 Mathematical modeling
• CPC Class Codes: B03B 5/00 Washing granular, powdered or lumpy materials; Wet separating separating by pneumatic tables or by pneumatic jigs B03B4/00
• CPC Class Codes: G01N 2030/582 micellar electrokinetic capillary chromatography [MECC]
• CPC Class Codes: G01N 30/0005 Field flow fractionation
• CPC Class Codes: G01N 30/02 Column chromatography
• CPC Class Codes: G01N 30/6095 Micromachined or nanomachined, e.g. micro- or nanosize
• CPC Class Codes: Y10S 366/01 Micromixers: continuous laminar flow with laminar boundary mixing in the linear direction parallel to the fluid propagation with or without conduit geometry influences from the pathway

Abstract

This invention provides an extraction device and a method for extracting desired particles from a sample stream containing the desired particles. The device has a sample stream inlet, an extraction stream inlet, and an extraction channel in fluid communication with the sample stream inlet and the extraction stream inlet. The extraction channel is for receiving a sample stream from the sample stream inlet in adjacent laminar flow with an extraction stream from the extraction stream inlet. A sequestering material within the extraction channel captures desired particles in the extraction stream. A by-product stream outlet in fluid communication with the extraction channel receives a by-product stream comprising at least a portion of the sample stream form which desired particles have been extracted. A product outlet in fluid communication with the extraction channel receives a product which has the sequestering material and at least a portion of the desired particles.

Claims

1. A device for enhancing differential transport of desired particles from a sample stream containing said desired particles into an extraction stream, said device comprising:

a. a sample stream inlet;

b. an extraction stream inlet;

c. a flow channel in fluid communication with said sample stream inlet and said extraction stream inlet for receiving a sample stream from said sample stream inlet in adjacent laminar flow with an extraction stream from said extraction stream inlet;

d. a sequestering material within said flow channel for capturing desired particles in said extraction stream; and

e. at least one outlet in fluid communication with said flow channel.

2. The device of claim 1 made by a microfabrication process.

3. A device of claim 1 wherein said sequestering material is an adsorbent material.

4. A device of claim 1 wherein said sequestering material is an absorbent material.

5. A device of claim 1 wherein said sequestering material is effectively non-diffusing in said device.

6. A device of claim 1 comprising desired particles reversibly bound to said sequestering material.

7. A device of claim 1 wherein said product contains greater than 50% of said desired particles in said sample stream.

8. A device of claim 1 wherein said product contains greater than 75% of said desired particles in said sample stream.

9. A device of claim 1 wherein substantially all of said desired particles are removed from said sample stream.

10. A device of claim 1 having a width of between about 2 micrometers and one meter.

11. A device of claim 1 having a width between about 5 micrometers and 5 centimeters.

12. A device of claim 1 having a width between about 10 micrometers and 100 micrometers.

13. An analytic system comprising a device of claim 1 in combination with means for detecting the presence of said desired particles captured by said sequestering material.

14. An analytic system comprising a device of claim 1 in combination with means for detecting the presence of an analyte in a by-product stream from which desired particles have been removed.

15. A system comprising a device of claim 1 in combination with means for removing said sequestering material with said captured desired particles from said product stream.

16. An analytic system comprising a device of claim 13 in combination with means for detecting the presence of an analyte in a product stream from which desired particles and sequestering material have been removed.

17. An apparatus containing a plurality of devices of claim 1.

18. A method for enhancing differential transport of at least a portion of desired particles from a sample stream comprising said desired particles into an extraction stream, said method comprising the steps of:

a) introducing said sample stream into the sample stream inlet of an extraction device comprising;

i) a sample stream inlet;

ii) an extraction stream inlet;

iii) a flow channel in fluid communication with said sample stream inlet and said extraction stream inlet for receiving a sample stream from said sample stream inlet in adjacent laminar flow with an extraction stream from said extraction stream inlet;

iv) a sequestering material within said flow channel for capturing desired particles in said extraction stream; and

v) at least one outlet in fluid communication with said flow channel;

b) introducing an extraction stream into the flowchannel of said extraction device; and

c) introducing into said extraction channel a sequestering material for capturing the desired particles such that the desired particles are captured by the sequestering material.

19. The method of claim 18 wherein said sample stream is blood and said desired particles are toxin particles.

20. The method of claim 18 wherein said desired particles are particles of a substance which interferes with analysis of said sample stream.

21. The method of claim 18 wherein said sequestering material comprises polymeric beads.

22. The method of claim 18 wherein said sequestering material comprises an enzyme which binds to said desired particles.

23. The method of claim 18 wherein said sequestering material is an absorbent material.

24. The method of claim 18 wherein said sequestering material is a substantially non-diffusing high molecular weight polymer.

25. The method of claim 18 wherein before performing steps a, b, and c, the device is pre-coated with a hydrophilic material by injecting the hydrophilic material into the sample stream inlet and extraction stream inlet.

26. The method of claim 18 wherein a major portion of said desired particles are transported from said sample stream into said extraction stream.