Comparative phenotype analysis for assessment of biological active compounds such as antimicrobials
First Claim
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1. A method for detecting the combined effect of at least two biologically active chemicals in vivo, comprising the steps of:
- a) providing;
i) a suspension comprising at least one cell and a first biologically active chemical, and ii) a testing device having at least two wells, wherein at least one well further comprises at least one subsequent biologically active chemical, and wherein said first and said subsequent biologically active chemicals are different compounds;
b) inoculating said suspension into said wells of said testing device; and
c) detecting a cellular response of said at least one cell in said wells.
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Abstract
The present invention relates to using multitest panels to improve the effectiveness, throughput, and efficiency of testing and commercial development of biologically active compounds, in particular those useful in human, animal, and plant health. In particular, the present invention provides phenotype microarrays suitable for testing biologically active compounds for their potential application in clinical, veterinary, and plant health.
36 Citations
22 Claims
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1. A method for detecting the combined effect of at least two biologically active chemicals in vivo, comprising the steps of:
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a) providing;
i) a suspension comprising at least one cell and a first biologically active chemical, and ii) a testing device having at least two wells, wherein at least one well further comprises at least one subsequent biologically active chemical, and wherein said first and said subsequent biologically active chemicals are different compounds;
b) inoculating said suspension into said wells of said testing device; and
c) detecting a cellular response of said at least one cell in said wells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method for examining the effects of at least two biologically active chemicals in vivo, comprising the steps of:
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a) obtaining a metabolic profile for a first biologically active chemical by detecting a response of a cell suspension incubated in the presence of a first biologically active chemical in a testing device comprising a plurality of wells each containing a different substrate;
b) obtaining a metabolic profile for at least one subsequent biologically active chemical by detecting a response of a cell suspension incubated in the presence of a subsequent biologically active chemical in a testing device comprising a plurality of wells each containing a different substrate; and
c) creating a database of said metabolic profiles. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
(e) obtaining a metabolic profile for a drug candidate by detecting a response of a cell suspension incubated in the presence of a drug candidate in a testing device comprising a plurality of wells each containing a different substrate; and
(f) determining whether said drug candidate falls into a group defined in step (d).
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Specification