Method and devices for detecting and enumerating microorganisms
First Claim
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1. An assay device, comprising a substrate having a plurality of microchannels therein, wherein said microchannels have at least one assay reagent for enumerating microorganisms coated thereon and the volume of one microchannel is different than the volume of another microchannel.
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Abstract
A method for detecting a microorganism in a test sample is described. The method involves distributing microvolumes of a sample to a plurality of microcompartments of a culture device, incubating for a time sufficient to permit at least one cell division cycle of the microorganism, then detecting the presence or absence of the microorganism in the microcompartments. Also disclosed are devices for carrying out these methods.
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8 Claims
- 1. An assay device, comprising a substrate having a plurality of microchannels therein, wherein said microchannels have at least one assay reagent for enumerating microorganisms coated thereon and the volume of one microchannel is different than the volume of another microchannel.
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