Method for analyzing a biological sample

• US 6,699,382 B2
• Filed: 09/04/2002
• Issued: 03/02/2004
• Est. Priority Date: 06/11/1998
• Status: Expired due to Term

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Watanabe, Motokazu, Ikeda, Shin, Yoshioka, Toshihiko, Nankai, Shiro
• Primary Examiner(s): TUNG, TA HSUNG
• Application Number:
  US10/235,451
• Publication Number:
  US 20030000834A1
• Time in Patent Office: 545 Days
• Field of Search: 204/403.01, 204/435, 204/418, 204/416, 204/403.04, 204/403.09, 204/403.1, 204/403.11, 204/403.14, 205/777.5, 205/778
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/001 Enzyme electrodes

Abstract

A biosensor enabling accurate measurement of the concentration of a substrate contained in a trace amount of a sample solution is disclosed. The electrochemical analysis element in accordance with the present invention which should be included in the biosensor comprises a sensor body having a hollow space which space is open at its both ends, an electrode system having a working electrode and a counter electrode, a reagent segment containing an enzyme, wherein the electrode system and the reagent segment are disposed on an inner wall of the hollow space.

Claims

1. A method for analyzing a biological sample, comprising (a) providing an electrochemical analysis element comprising a sensor body having a hollow space which space is open at both of its ends, both ends being arranged along an axis of the element, an electrode system including a working electrode and a counter electrode and a reagent segment containing an enzyme, wherein said electrode system and said reagent segment are disposed on an inner wall of said hollow space, (b) pressing one of the open ends of the hollow space against a skin portion of a host from whom a sample is to be analyzed, and (c) introducing the sample into the hollow space through a filter disposed on the one open end.

2. The method according to claim 1, wherein the working electrode is positioned opposite to the counter electrode on the inner wall of the hollow space.

3. The method according to claim 1, wherein the reagent segment further comprises an electron acceptor.

4. The method according to claim 1, wherein the reagent segment is disposed on the working electrode.

5. The method according to claim 1, wherein the sample is introduced into the hollow space by capillary action.

6. The method according to claim 1, wherein the hollow space has a capillary size to facilitate supply of the sample by capillary transport from one end of the space to the electrodes.

7. The method according to claim 1, further comprising (d) providing a body fluid leaking instrument and (e) actuating the body fluid leaking instrument to damage the skin portion of the host to release the sample to be analyzed.

8. The method according to claim 7, wherein the body fluid leaking instrument is selected from the group consisting of a laser beam emitting device and a lancet.

9. The method according to claim 7, wherein the hollow space has a size that allows for passage of the body fluid leaking instrument from the one open end to the other open end.

10. The method according to claim 1, wherein the sample to be analyzed comprises whole blood and the filter filters out red blood cells.

11. A method for analyzing a biological sample according to claim 1, wherein each open end is covered with a film.

12. The method according to claim 11, wherein the film comprises a sealing material.

13. The method according to claim 12, wherein the film is selected from the group consisting of resin and aluminum laminate.

14. A method for analyzing biological samples according to claim 1, wherein the sensor body has a plurality of said hollow spaces, and step (b) comprises selectively pressing one of the open ends of each hollow space against a skin portion of a host from whom samples are to be analyzed, such that samples are directly and selectively introduced into different ones of the hollow spaces.

15. The method according to claim 14, wherein the sensor body is an insulating disc member and the hollow spaces are arranged along the periphery of the disc member.

16. The method according to claim 14, wherein the analysis element has a cylindrical shape.

17. The method according to claim 14, wherein each hollow space has a circular or semicircular cross sectional shape.

18. The method according to claim 14, wherein the analysis element has a substantially uniform length.