Endotoxin adsorbent, and a method of removing endotoxin by using the same

• US 6,699,386 B2
• Filed: 03/01/2002
• Issued: 03/02/2004
• Est. Priority Date: 03/14/2001
• Status: Active Grant

• Current Assignee: JNC Corporation (Chisso Corporation)
• Original Assignee: Chisso Corporation
• Inventors: Hirayama, Chuichi, Kunitake, Masashi, Todokoro, Masami, Sakata, Masayo
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US10/085,005
• Publication Number:
  US 20020130082A1
• Time in Patent Office: 732 Days
• Field of Search: 210/635, 210/656, 210/679, 210/791, 210/198.2, 210/263, 210/502.1, 210/506, 502/401, 502/402, 502/404
• US Class Current: 210/198.2
• CPC Class Codes: B01J 20/24 Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
• CPC Class Codes: B01J 20/26 Synthetic macromolecular compounds
• CPC Class Codes: B01J 20/262 obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds per se C08G
• CPC Class Codes: B01J 20/265 modified or post-treated polymers polymer carriers or substrates subjected to further impregnating or coating B01J20/3208
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3217 Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/3255 comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures
• CPC Class Codes: B01J 20/3272 Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds

Abstract

This invention provides an adsorbent having a high ability to adsorb endotoxin selectively and a method of adsorbing endotoxin. The adsorbent comprises a basic substance bonded to a base material by means of a crosslinking agent.

Claims

1. An endotoxin adsorbent with an exclusion limit molecular weight of 6000 or less as determined by polysaccharide standards, which comprises a basic substance bonded to a base material by means of a crosslinking agent.

2. The endotoxin adsorbent according to claim 1, wherein the base material is a polysaccharide selected from cellulose, agarose, chitosan, chitin, starch and mannan, a derivative of said polysaccharide, a polymer of a monomer selected from acrylamide, methacrylamide, acrylic acid, acrylate, methacrylic acid, methacrylate, vinyl acetate, vinyl chloride, styrene and an allyl compound, or a copolymer of two or more of these monomers.

3. The endotoxin adsorbent according to claim 1, wherein the base material is a polysaccharide selected from cellulose, agarose, chitosan, chitin, starch and mannan, a derivative of said polysaccharide, a polymer of a monomer selected from acrylic acid, acrylate, methacrylic acid, methacrylate, vinyl acetate, vinyl chloride, styrene and an allyl compound, or a copolymer of two or more of these monomers.

4. The endotoxin adsorbent according to claim 1, wherein the base material is cellulose or a derivative thereof.

5. The endotoxin adsorbent according to any one of claims 1 to 4, wherein the base material is porous cellulose or a derivative thereof having an exclusion limit molecular weight of 6000 or less as determined by saccharide standards.

6. The endotoxin adsorbent according to any one of claims 1 to 4, wherein the base material is porous cellulose or a derivative thereof having an exclusion limit molecular weight of 2000 or less as determined by saccharide standards.

7. The endotoxin adsorbent according to claim 1, wherein the basic substance is at least one member selected from ε

-poly-L-lysine, α

-poly-L-lysine, poly-histidine, poly-arginine, poly-tryptophan, polyornithine, poly-2,4-diaminobutyric acid, poly-2,3-diaminopropionic acid, protamine, polyallylamine, an allylamine-diallylamine copolymer, polydiallylamine, polyvinylamine, polymyxin, streptomycin, amikacin, and kanamycin.

8. The endotoxin adsorbent according to claim 1, wherein the basic substance is ε

-poly-L-lysine or α

-poly-L-lysine.

9. The endotoxin adsorbent according to claim 1, wherein the crosslinking agent is at least one crosslinking agent selected from hexamethylene diisocyanate, m-xylene diisocyanate, toluene-2,4-diisocyanate, diglycidyl ether, epichlorohydrin and halogenated epichlorohydrin.