Polymerase chain reaction system
First Claim
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1. A polymerase chain reaction DNA amplification and detection system, comprising:
- one or more sample chamber modules for receiving a biological sample, each of said sample chamber modules supporting at least two independent measurements on said biological sample, and parallel interrogation ports operatively connected to said sample chamber modules.
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Abstract
A portable polymerase chain reaction DNA amplification and detection system includes one or more chamber modules. Each module supports a duplex assay of a biological sample. Each module has two parallel interrogation ports with a linear optical system. The system is capable of being handheld.
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Citations
40 Claims
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1. A polymerase chain reaction DNA amplification and detection system, comprising:
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one or more sample chamber modules for receiving a biological sample, each of said sample chamber modules supporting at least two independent measurements on said biological sample, and parallel interrogation ports operatively connected to said sample chamber modules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. A method of polymerase chain reaction DNA amplification and detection, comprising the steps of:
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placing a biological sample in a sample chamber module that supports at least two independent measurements on said biological sample, and directing light energy into said biological sample through parallel interrogation ports operatively connected to said sample chamber module. - View Dependent Claims (35, 36, 37, 38, 39, 40)
heating said sample using a heating unit in a silicon base, and cooling said sample.
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37. The method of polymerase chain reaction DNA amplification and detection of claim 36, including forcing cooling air through a sleeve reaction chamber to cooling said biological sample.
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38. The method of polymerase chain reaction DNA amplification and detection of claim 34, wherein said sample chamber module includes an individual sample chamber and including the step of temporarily overheating said sample chamber for the purpose of having said biological sample achieve and hold a desired temperature as rapidly as possible.
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39. The method of polymerase chain reaction DNA amplification and detection of claim 34, including the step of:
determining that an assay of said sample is positive based on a positive calling algorithm.
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40. The method of polymerase chain reaction DNA amplification and detection of claim 39, including the step of:
displaying in real-time the degree of certainty or of reliability for each said assay.
Specification