Biosensor arrays and methods
First Claim
1. A method for fabricating a surface detector array device, comprising the steps of:
- providing a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions, wherein said bilayer-compatible surface regions and said bilayer barrier regions are formed of different materials;
providing a first suspension of lipid-bilayer vesicles having a first composition;
providing a second suspension of lipid-bilayer vesicles having a second composition;
transferring an aliquot of said first suspension to a first of said plurality of distinct bilayer-compatible surface regions;
transferring an aliquot of said second suspension to a second of said plurality of distinct bilayer-compatible surface regions;
incubating said substrate with said first and said second suspension to form a first lipid bilayer expanse stably localized above said first distinct bilayer-compatible surface region, and a second lipid bilayer expanse stably localized above said second distinct bilayer-compatible surface region, wherein each of said expanses is localized above each of said surface regions in the absence of covalent linkages between each of said lipid bilayer expanses and each of said bilayer-compatible surface regions, and is separated therefrom by an aqueous film interposed between said bilayer-compatible surface regions and said corresponding lipid bilayer expanses; and
establishing a bulk aqueous phase above said lipid bilayer expanses.
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Abstract
A surface detector array device suitable for use with a biosensor is disclosed. The device is formed of a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions. The bilayer-compatible surface regions carry on them, separated by a film of aqueous, supported fluid bilayers. The bilayers may contain selected receptors or biomolecules. A bulk aqueous phase covers the bilayers on the substrate surface. Multiplexed assays using the surface detector array device of the present invention are disclosed, as are automated methods for making the surface detector array device that enable formation of arrays wherein the composition of the individual, addressable bilayer regions is unrestricted.
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Citations
34 Claims
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1. A method for fabricating a surface detector array device, comprising the steps of:
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providing a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions, wherein said bilayer-compatible surface regions and said bilayer barrier regions are formed of different materials;
providing a first suspension of lipid-bilayer vesicles having a first composition;
providing a second suspension of lipid-bilayer vesicles having a second composition;
transferring an aliquot of said first suspension to a first of said plurality of distinct bilayer-compatible surface regions;
transferring an aliquot of said second suspension to a second of said plurality of distinct bilayer-compatible surface regions;
incubating said substrate with said first and said second suspension to form a first lipid bilayer expanse stably localized above said first distinct bilayer-compatible surface region, and a second lipid bilayer expanse stably localized above said second distinct bilayer-compatible surface region, wherein each of said expanses is localized above each of said surface regions in the absence of covalent linkages between each of said lipid bilayer expanses and each of said bilayer-compatible surface regions, and is separated therefrom by an aqueous film interposed between said bilayer-compatible surface regions and said corresponding lipid bilayer expanses; and
establishing a bulk aqueous phase above said lipid bilayer expanses. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. An apparatus for fabricating a surface detector array device, comprising:
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a platform for receiving a substrate, said substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions, wherein said bilayer-compatible surface regions and said bilayer barrier regions are formed of different materials;
a source of a first suspension of lipid-containing vesicles or liposomes having a first composition;
a source of a second suspension of lipid-containing vesicles or liposomes having a second composition;
means for transferring an aliquot of said first suspension to a first of said plurality of distinct bilayer-compatible surface regions and for transferring an aliquot of said second suspension to a second of said plurality of distinct bilayer-compatible surface regions; and
a source of a bulk aqueous phase used to cover said fabricated surface detector array device. - View Dependent Claims (19, 20, 21, 34)
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22. A multiplexed assay, comprising the steps of:
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providing a surface detection array device, said device comprising a substrate having a surface defining a plurality of distinct bilayer-compatible surface regions separated by one or more bilayer barrier regions, said bilayer-compatible surface regions and said bilayer barrier regions being formed of different materials, a first lipid bilayer expanse having a first composition and stably localized above a first of said plurality of distinct bilayer-compatible surface regions, a second lipid bilayer expanse having a second composition different from said first composition and stably localized above a second of said plurality of distinct bilayer-compatible surface regions, wherein each of said expanses is localized above each of said surface regions in the absence of covalent linkages between each of said lipid bilayer expanses and each of said bilayer-compatible surface regions, and is separated therefrom by an aqueous film interposed between said bilayer-compatible surface regions and said corresponding lipid bilayer expanses;
contacting said device with a bulk aqueous phase comprising a test agent; and
assaying an interaction between said test agent and said first composition and an interaction between said test agent and said second composition. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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Specification