Method of determining autoregulatory and perfusion status

• US 6,701,169 B1
• Filed: 11/06/2001
• Issued: 03/02/2004
• Est. Priority Date: 04/30/1999
• Status: Expired due to Fees

• Current Assignee: The UA Research Foundation, UAB Research Foundation (University of Alabama System)
• Original Assignee: UAB Research Foundation (University of Alabama System)
• Inventors: Denninghoff, Kurt R.
• Primary Examiner(s): WINAKUR, ERIC FRANK
• Application Number:
  US10/018,261
• Time in Patent Office: 847 Days
• Field of Search: 600/310, 600/318, 600/320, 600/322, 600/323, 600/324, 600/328, 600/340
• US Class Current: 600/320
• CPC Class Codes: A61B 3/1241 specially adapted for observation of ocular blood flow, e.g. by fluorescein angiography
• CPC Class Codes: A61B 5/14555 specially adapted for the eye fundus

Abstract

A method of determining the autoregulatory status of a subject by obtaining a measurement of a selected parameter of the retinal blood vessels in a non-stimulated subject, administering to the subject a preselected stimulus, obtaining a measurement of the selected parameter of the retinal blood vessels of the subject in response to the administration of the selected stimulus, and determining the ratio of the measurement for the selected parameter in the non-stimulated retinal blood vessels to the measurement of the selected parameter for the retinal blood vessels following the administration of its selected stimulus whereby the ratio provides an indicator of the autoregulatory function or status of the subject.

Claims

1. A method of determining the autoregulatory status of a subject, said method comprising the steps of:

obtaining a measurement of a selected parameter of the retinal blood vessels in a non-stimulated subject;

administering to the subject a preselected stimulus;

obtaining a measurement of the selected parameter of the retinal blood vessels of the subject in response to the administration of the selected stimulus; and

determining the ratio of the measurement for the selected parameter in the non-stimulated retinal blood vessels to the measurement of the selected parameter for the retinal blood vessels following the administration of the selected stimulus whereby the ratio provides an indicator of the autoregulatory function or status of the subject.

2. A method according to claim 1, wherein said obtaining steps further comprise measuring the selected parameter with a laser scanning device.

3. A method according to claim 1, wherein the selected parameter comprises oxygen saturation of the retinal blood vessels.

4. A method according to claim 1, wherein the selected parameter comprises retinal blood vessel diameter.

5. A method according to claim 1, wherein the preselected parameter includes both oxygen saturation and blood vessel diameter in retinal blood vessels.

6. A method according to claim 1, wherein the preselected stimulus is 100% oxygen.

7. A method according to claim 1, wherein the preselected stimulus is selected from the group consisting of glucose, lactic acid, and carbon dioxide.

8. A method according to claim 1, wherein said determining step further comprises calculating the autoregulatory state using the equation:

9. A method of diagnosing and monitoring glaucoma in a subject by assessing autoregulatory function, said method comprising the steps of:

measuring the venous oxygen saturation level of retinal blood vessels of a subject under normoxic conditions;

measuring the venous oxygen saturation level of the retinal blood vessels of the subject under hyperoxic conditions; and

determining the ratio of the normoxic venous oxygen saturation level to the hyperoxic venous oxygen saturation level, wherein the ratio provides an indicator of the autoregulatory or status of the subject which can be used to diagnose and/or monitor the subject for glaucoma.

10. A method according to claim 9 further including the step of administering oxygen to the subject to induce the hyperoxic condition.

11. A method according to claim 9, wherein said determining step further comprises calculating the autoregulatory state using the equation:

12. A method of monitoring blood loss in a subject, said method comprising the steps of:

measuring of retinal venous oxygen saturation;

measuring retinal venous diameter; and

calculating the ratio of the measurement of retinal venous oxygen saturation to retinal venous diameter wherein the ratio provides an indicator of the amount of blood loss in the subject.