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Apparatus and methods for correcting for variable velocity in microfluidic systems

  • US 6,703,205 B2
  • Filed: 03/19/2002
  • Issued: 03/09/2004
  • Est. Priority Date: 06/09/1997
  • Status: Expired due to Term
First Claim
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1. A method of detecting the concentration of a product, rate of product formation, or the amount of the product produced by contacting at least a first and second component of a reaction comprising:

  • contacting the first and second reactant in a microfluidic channel, wherein the first reactant comprises a detectable label, thereby producing a product comprising the detectable label, which product has a different electrokinetic mobility than the first or second reactant, wherein flux is conserved in the microfluidic channel, whereby

    h=1m






    CRh

    Uh
    =

    i=1n






    CPi

    Ui
    embedded image

    wherein C refers to mass concentration, R refers to the reactant species present in the microfluidic channel, P refers to product species produced in the microfluidic channel, U refers to velocity, m is the number of species prior to said contacting, and n is the number of species after said contacting;

    flowing the product and any first or second reactant remaining in the channel subsequent to said contacting step past a detector, wherein the label on the first reactant and the label on the product comprise the same detectable moiety; and

    , determining at least one of;

    concentration of the product, rate of product formation, or amount of product produced, wherein flux is conserved, whereby the sum of the concentration of the reactants and products times the respective velocity of reactants and products is constant.

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