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Cellular detection by providing high energy phosphate donor other than ADP to produce ATP

  • US 6,703,211 B1
  • Filed: 09/15/1999
  • Issued: 03/09/2004
  • Est. Priority Date: 03/13/1998
  • Status: Expired due to Fees
First Claim
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1. A method for determining the presence of cells in an aqueous sample composition that comprises the steps of:

  • (a) admixing said aqueous sample composition with a high energy phosphate donor other than ADP to form a reaction mixture, said cells, when present, being treated to form ATP in said reaction mixture;

    (b) maintaining said reaction mixture for a time period sufficient for enzymes endogenous to the cells to convert adenosine present to ATP; and

    (c) assaying for the presence of ATP, the presence of ATP indicating the presence of cells in said aqueous sample.

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