Localized molecular and ionic transport to and from tissues

• US 6,706,032 B2
• Filed: 06/07/2001
• Issued: 03/16/2004
• Est. Priority Date: 06/08/2000
• Status: Expired due to Fees

• Current Assignee: Massachusetts Institute of Technology, The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: Massachusetts Institute of Technology, The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Anderson, R. Rox, Weaver, James C., Gonzalez, Salvador, Gowrishankar, T. R., Gift, Elizabeth A., Herndon, Terry O
• Primary Examiner(s): Casler, Brian L.
• Assistant Examiner(s): Maynard, Jennifer
• Application Number:
  US09/878,155
• Publication Number:
  US 20020065533A1
• Time in Patent Office: 1,013 Days
• Field of Search: 604/500, 604/501, 604/518, 604/522, 604/521, 604/57, 604/58, 604/68-72, 604/19-20, 604/22, 604/48, 606/131, 606/167, 606/184, 606/185, 128/898, 47/575
• US Class Current: 604/500
• CPC Class Codes: A61M 2037/0007 having means for enhancing the permeation of substances through the epidermis, e.g. using suction or depression, electric or magnetic fields, sound waves or chemical agents
• CPC Class Codes: A61M 37/00 Other apparatus for introducing media into the body for reproduction or fertilisation A61B17/425; apparatus for iontophoresis or cataphoresis A61N1/30; Percutany, i.e. introducing medicines into the body by diffusion through the skin salt baths A61H33/04
• CPC Class Codes: A61M 5/427 Locating point where body is to be pierced, e.g. vein location means using ultrasonic waves, injection site templates
• CPC Class Codes: A61N 1/044 Shape of the electrode
• CPC Class Codes: A61N 1/30 Apparatus for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body, or cataphoresis
• CPC Class Codes: A61N 1/325 for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body electrodes for external use A61N1/0428

Abstract

The present invention relates to methods and devices used for the formation of microconduits in a tissue. The term “microconduit” refers to a small opening, channel, or hole into, or through, a tissue, that allows transfer of materials by liquid flow, and by electrophoresis, the microconduit being formed upon impact of a plurality of accelerated microparticles with the surface of the tissue. A method is described for forming at least one microconduit in tissue including the steps of: accelerating a plurality of microparticles to a velocity that causes the microparticles to penetrate a region of tissue surface upon impingement of the microparticles on the tissue surface; and directing the microparticle towards the region of tissue surface, thereby causing the microparticles to penetrate the tissue and form a microconduit in the tissue. According to an embodiment, microparticles are accelerated by being hit with a moving, solid surface. In another embodiment, microparticles are accelerated by a flowing gas or liquid. Also described are methods and devices for using microconduits to deliver therapeutic molecules and ions into tissue, or for extraction of chemical analytes out of tissue. Also described is a method of nail piercing to accommodate jewelry.

Claims

1. A method for forming at least one microconduit in a tissue, comprising the steps of:

a) accelerating a plurality of microparticles to a velocity that causes the microparticles to penetrate a region of a tissue surface upon impingement of the microparticles on the tissue surface;

b) directing the microparticles towards the region of tissue surface, thereby causing the microparticles to penetrate the tissue; and

c) scissioning the tissue with the impinging microparticles, thereby forming a plurality of free microtissue particles, and thereby forming a microconduit.

2. The method of claim 1, wherein the microparticles are accelerated by a flowing gas.

3. The method of claim 2, wherein the flowing gas is at a pressure greater than about one atmosphere absolute.

4. The method of claim 2, wherein the flowing gas is at a pressure of less than about one atmosphere absolute.

5. The method of claim 1, wherein the microparticles are accelerated by means of a flowing liquid.

6. The method of claim 5, wherein the flowing liquid is at a pressure greater than about one pound per square inch.

7. The method of claim 5, wherein the flowing liquid is at a temperature of below about 20°

C.

8. The method of claim 1, wherein the microparticles are accelerated by contacting the microparticles with a moving, solid surface.

9. The method of claim 8, wherein the moving solid surface includes a rotating impeller.

10. The method of claim 1, wherein the microparticles are directed through at least one microhole in a mask at the tissue surface, the mask comprising a membrane.

11. The method of claim 10, wherein the membrane is conformable to the tissue surface.

12. The method of claim 1, wherein a plurality of microparticles are accelerated and directed to the region of tissue surface, and wherein the microparticles are collimated to form a beam of collimated microparticles.

13. The method of claim 12, wherein the collimated beam of microparticles is scanned over the region of tissue surface.

14. The method of claim 13, wherein the collimated beam of microparticles is gated on and off.

15. The method of claim 1, wherein the microparticles are accelerated to a velocity sufficient to cause tissue material to be microscissioned from the tissue by impingement of the microparticles on the tissue surface.

16. The method of claim 15, wherein the tissue material that is microscissioned is ejected from the tissue surface.

17. The method of claim 1, wherein the microparticles are accelerated to a velocity sufficient to cause tissue material to be compacted when the microparticles penetrate the tissue.

18. The method of claim 1, wherein the microparticles impinge upon the region of tissue surface which has an area in a range of between about 100 square micrometers and about two million square micrometers.

19. The method of claim 1, further including the step of modifying at least one physical property of the region of tissue surface.

20. The method of claim 1, further including the step of applying at least one electrical pulse to cause electroporation of at least one lipid-containing membrane of the region of tissue surface.

21. The method of claim 20, wherein the electrical pulse is applied following formation of the microconduit.

22. The method of claim 1, further including the step of applying a direct current voltage to the microconduit to produce iontophoresis.

23. The method of claim 22, wherein the direct current voltage applied to the microconduit is pulsed.

24. The method of claim 1, wherein the microparticles are not soluble in water.

25. The method of claim 1, wherein the microparticles are soluble in water.

26. The method of claim 25, wherein the microparticles include a therapeutically effective substance.

27. The method of claim 1, wherein the microparticles include aluminum oxide.

28. The method of claim 1, wherein the microparticles include sodium bicarbonate.

29. The method of claim 1, wherein the microparticles include urea.

30. The method of claim 1, wherein the microparticles include solid carbon dioxide.

31. The method of claim 1, wherein the microparticles include solid water.

32. The method of claim 1, wherein the melting point of the microparticles is less than about 33°

C.

33. The method of claim 32, wherein the microparticles include at least one therapeutically effective substance.

34. The method of claim 2, wherein the flowing gas includes air.

35. The method of claim 2, wherein the flowing gas is at a temperature of below about 20°

C.

36. The method of claim 2, wherein the flowing gas includes an inert gas.

37. The method of claim 1, further including the step of applying a chemical agent to the microconduit that affects a rate of recovery of the microconduit.

38. The method of claim 37, wherein the chemical agent includes a calcium ion.

39. The method of claim 37, wherein the chemical agent includes 5-fluorouracil.

40. The method of claim 37, wherein the chemical agent is selected from the group consisting of retinoids, surfactants, and antigents.

41. The method of claim 40, wherein the chemical agent includes retinoic acid.

42. The method of claim 37, further including the step of directly applying pressure to the microconduit through a column containing the chemical agent, the column sealed to the tissue around the microconduit.

43. The method of claim 1, further including the step of testing for the presence of blood within the microconduit.

44. The method of claim 43, wherein the test employs optical means.

45. The method of claim 44, wherein the optical means includes image analysis.

46. A method for forming at least one opening in the stratum corneum of skin comprising the steps of:

a) accelerating a plurality of microparticles to a velocity that causes the microparticles to penetrate a region of a skin surface upon impingement of the microparticles on the skin surface;

b) directing the microparticles towards the region of skin surface, thereby causing the microparticles to penetrate the skin; and

c) scissioning the skin with the impinging microparticles, thereby forming a plurality of free microtissue particles, and thereby forming a microconduit in the skin.

47. The method of claim 46, wherein the microparticles include at least one therapeutically effective substance.

48. The method of claim 46, wherein the microparticles are accelerated by a flowing gas.

49. The method of claim 48, wherein the flowing gas is at a pressure greater than about one atmosphere absolute.

50. The method of claim 48, wherein the flowing gas is at a pressure of less than about one atmosphere absolute.

51. The method of claim 46, wherein the microparticles are accelerated by means of a flowing liquid.

52. The method of claim 51, wherein the flowing liquid is at a pressure greater than about one pound per square inch.

53. The method of claim 51, wherein the flowing liquid is at a temperature of below about 20°

C.

54. The method of claim 46, wherein the microparticles are accelerated by contacting the microparticles with a moving, solid surface.

55. The method of claim 54, wherein the moving solid surface includes a rotating impeller.

56. The method of claim 46, wherein the microparticles are directed through at least one microhole in a solid mask at the skin surface.

57. The method of claim 46, wherein the microparticles that are directed to the region of skin surface are collimated to form a beam of collimated microparticles.

58. The method of claim 57, wherein the beam of collimated microparticles is scanned over the region of skin surface.

59. The method of claim 58, wherein the beam of collimated microparticles is gated on and off.

60. The method of claim 46, wherein the microparticles are accelerated to a velocity sufficient to cause skin material to be microscissioned from the skin by impingement of the microparticles on the skin surface.

61. The method of claim 60, wherein the skin material that is microscissioned is ejected from the skin surface.

62. The method of claim 46, wherein the microparticles are accelerated to a velocity sufficient to cause skin material to be compacted when the microparticles penetrate the skin.

63. The method of claim 46, wherein the microparticles impinge upon a region of skin surface having an area equal to between about 1000 square micrometers and about 100,000 square micrometers.

64. The method of claim 46, further including the step of modifying at least one physical property of the region of skin surface.

65. The method of claim 46, further including the step of applying at least one electrical pulse to cause electroporation of at least one lipid-containing membrane of the skin to thereby cause formation of at least one aqueous pathway.

66. The method of claim 65, wherein the electrical pulse is applied following formation of the microconduit.

67. The method of claim 65, further comprising the step of applying at least one modifying agent that alters the aqueous pathway.

68. The method of claim 46, further including the step of applying a direct current voltage to the microconduit to produce iontophoresis.

69. The method of claim 68, wherein the direct current voltage applied to the microconduit is pulsed.

70. The method of claim 46, wherein the microconduit fully penetrates a stratum corneum layer of the skin.

71. The method of claim 70, wherein the microconduit further penetrates the epidermis.

72. The method of claim 70, wherein the microconduit further penetrates the dermis.

73. The method of claim 46, further comprising the step of making at least one measurement of the amount of water vapor in a gas exiting from the microconduit during formation of the microconduit.

74. The method of claim 46, further including the step of making at least one measurement of electrical impedance of the region of skin surface to monitor formation of the microconduit during impingement by the microparticles.

75. A method of delivering a therapeutic molecule or ion to tissue, comprising the steps of:

a) accelerating a plurality of microparticles to a velocity that causes the microparticles to penetrate a region of a tissue surface upon impingement of the microparticles on the tissue surface;

b) directing the microparticles towards the region of tissue surface, thereby causing the microparticles to penetrate the tissue;

c) scissioning the tissue with the impinging microparticles, thereby forming a plurality of free microtissue particles, and thereby forming a microconduit; and

d) administering at least one therapeutic molecule or ion by directing the therapeutic molecule or ion into at least one microconduit, thereby delivering a therapeutic molecule or ion to tissue.

76. The method of claim 75, comprising the further step of directly applying pressure to the microconduit through a column containing the therapeutic molecule or ion, the column sealed to the tissue around the microconduit.

77. The method of claim 76, wherein the pressure that is applied is a pressure gradient.

78. The method of claim 75, wherein the therapeutic molecule or ion is in a controlled release material.

79. The method of claim 75, wherein the therapeutic molecule or ion is supplied within a hydrogel, and the hydrogel is administered by directing the hydrogel into the microconduit, thereby delivering the therapeutic molecule or ion to the tissue.

80. The method of claim 75, wherein the therapeutic molecule or ion is an immunizing material.

81. The method of claim 75, wherein the therapeutic molecule or ion is a nucleic acid or a modified nucleic acid.

82. The method of claim 81, wherein the nucleic acid is DNA.

83. A method of transdermal delivery of a therapeutic molecule or ion, comprising the steps of:

a) accelerating a plurality of non-drug containing microparticles to a velocity that causes the microparticles to completely penetrate a region of a skin surface upon impingement of the microparticles on the skin surface;

b) directing the microparticles towards the region of the skin surface, thereby causing the microparticles to penetrate the skin;

c) scissioning the skin with the impinging microparticles, thereby forming a plurality of free microtissue particles, and thereby forming one or more microconduits; and

d) administering at least one therapeutic molecule or ion by directing the therapeutic molecule or ion into at least one of said microconduits, thereby delivering therapeutic molecule or ion through the stratum corneum.

84. The method of claim 83, comprising the further step of directly applying pressure to the microconduit through a column containing the therapeutic molecule or ion, the column sealed to the tissue around the microconduit.

85. The method of claim 83, wherein the therapeutic molecule or ion is in a controlled release material.

86. The method of claim 83, wherein the therapeutic molecule or ion is supplied within a hydrogel, and the hydrogel is administered by directing the hydrogel into at least one microconduit, thereby delivering a therapeutic molecule or ion through the stratum corneum.

87. The method of claim 83, further including the step of applying an electric field oriented in a direction to cause the therapeutic molecule or ion to migrate from the microconduit into the skin and parallel to a major plane of the region of skin surface.

88. The method of claim 83, further including the step of applying a stimulus to the skin that causes uptake of the therapeutic molecule or ion into at least one cell within the skin.

89. The method of claim 88, wherein the cell is a dendritic cell.

90. The method of claim 88, wherein the therapeutic molecule or ion is DNA or an anti-neoplastic drug.