Open capillary column and manufacturing method thereof

• US 6,706,177 B2
• Filed: 04/08/2002
• Issued: 03/16/2004
• Est. Priority Date: 02/21/2000
• Status: Expired due to Fees

• Current Assignee: Chemco Scientific Co., Ltd., Motonori Munesue, Takao Tsuda
• Original Assignee: Chemco Scientific Co., Ltd., Motonori Munesue, Takao Tsuda
• Inventors: Kitagawa, Shinya, Munesue, Motonori, Tsuda, Takao
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US10/119,132
• Publication Number:
  US 20030189001A1
• Time in Patent Office: 708 Days
• Field of Search: 95/88, 961/01, 210/635, 210/656, 210/658, 210/198.2, 210/198.3, 210/502.1
• US Class Current: 210/198.2
• CPC Class Codes: B01J 20/28054 characterised by their surface properties or porosity
• CPC Class Codes: B01J 20/28097 being coated, filled or plugged with specific compounds
• CPC Class Codes: B01J 20/282 Porous sorbents ion exchange B01J39/00 - B01J41/00
• CPC Class Codes: B01J 20/286 Phases chemically bonded to a substrate, e.g. to silica or to polymers
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: B01J 2220/84 Capillaries
• CPC Class Codes: G01N 2030/525 surface properties, e.g. porosity
• CPC Class Codes: G01N 2030/567 coating
• CPC Class Codes: G01N 30/52 Physical parameters
• CPC Class Codes: G01N 30/6078 Capillaries

Abstract

In an open capillary column, a stationary phase is formed on the inner wall of a capillary tube. This stationary phase comprises a number of pores on the surface of the capillary tube, and each of pore has an expanded hollow in the stationary phase. A manufacturing method of an open capillary column comprises the steps of giving alkaline treatment to an inner wall of an open capillary tube, applying one of oligo silica, oligo zirconia and oligo titania on the inner wall, filling the open capillary tube with alkaline solution to form one of a silica layer, a zirconia layer and titania layer, discharging the alkaline solution and heat-treating the capillary tube to form a base of a stationary phase. The open capillary column of the present invention comprises a stationary phase having a very large surface area. It is useful for a separation analysis field such as liquid chromatography and electrochromatography.

Claims

1. An open capillary column comprising an open capillary tube having an inner wall with a stationary phase, a surface of which being provided with a number of pores, each of which have an expanded hollow in the stationary phase.

2. An open capillary column according to claim 1, wherein the open capillary tube is a fused silica capillary tube.

3. An open capillary column according to claim 1, wherein the open capillary tube is obtainable by cutting an open capillary tube material with an arbitrary proper length.

4. An open capillary column according to claim 1, wherein oligo silica is a base material of the stationary phase.

5. An open capillary column according to claim 4, wherein the stationary phase is a chemically modified stationary phase obtained by giving chemical modification to at least one of the surface of the stationary phase and expanded hollows in the stationary phase.

6. An open capillary column according to claim 4, wherein the surface of the stationary phase and the expanded hollows in the stationary phase are subjected to different chemical modification to obtain the chemically modified stationary phase.

7. An open capillary column according to claim 1, wherein oligo zirconia is a base material of the stationary phase.

8. An open capillary column according to claim 1, wherein oligo titania is a base material of the stationary phase.

9. An open capillary column according to claim 1, wherein the stationary phase is a chemically modified stationary phase obtained by giving chemical modification to at least one of the surface of the stationary phase and expanded hollows in the stationary phase.

10. An open capillary column according to claim 9, wherein a functional group of chemical modification is a group selected from the group consisting of alkyl group, γ

-aminopropyl group, cyanoethyl group, cyclodextrin group and ion exchange group.

11. An open capillary column according to claim 1, wherein the surface of the stationary phase and the expanded hollows in the stationary phase are subjected to different chemical modifications to obtain the chemically modified stationary phase.

12. An open capillary column according to claim 11, wherein a functional group of chemical modification is a group selected from the group consisting of alkyl group, γ

-aminopropyl group, cyanoethyl group, cyclodextrin group and ion exchange group.