Systems and devices for photoelectrophoretic transport and hybridization of oligonucleotides
First Claim
1. A device for effecting the photoelectric transport of charged materials in a liquid environment, comprising:
- a substrate having a first face and a second face, the substrate capable of generating a photocurrent;
a conductor contacting at least a portion of the first face of the substrates a permeation layer supported on the second face, wherein the permeation layer comprises agarose;
attachment entities coupled to the permeation layer; and
a light source disposed to illuminate at least a portion of the substrate.
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Accused Products
Abstract
A platform for photoelectrophoretic transport and electronic hybridization of fluorescence labeled DNA oligonucleotides in a low conductivity electrolyte is described. A chemically stabilized semiconductor photodiode or photoconductor surface is coated with a streptavidin-agarose permeation layer. Micro-illumination of the surface generates photo-electrochemical currents that are used to electrophoretically transport and attach capture strands, preferably biotinylated DNA, to arbitrarily selected locations. The same process is then used to transport and electronically hybridize fluorescence labeled DNA target strands to the previously attached capture strands. Signal detection is accomplished either by a fluorescence scanner or a CCD camera. This represents a flexible electronic DNA assay platform that need not rely on pre-patterned microelectronic arrays.
145 Citations
49 Claims
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1. A device for effecting the photoelectric transport of charged materials in a liquid environment, comprising:
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a substrate having a first face and a second face, the substrate capable of generating a photocurrent;
a conductor contacting at least a portion of the first face of the substrates a permeation layer supported on the second face, wherein the permeation layer comprises agarose;
attachment entities coupled to the permeation layer; and
a light source disposed to illuminate at least a portion of the substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A device for effecting the photoelectric transport of charged materials in a liquid environment, comprising:
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a photoconductor substrate;
a permeation layer supported on the substrate, wherein the permeation layer comprises agarose;
attachment entities coupled to the permeation layer; and
a light source disposed to illuminate at least a portion of the substrate. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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39. The method for the photoelectric transport of charged materials in a liquid environment, comprising the steps of:
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providing a photoconductor substrate with a permeation layer disposed thereon, wherein the permeation layer comprises agarose;
contacting a charged material in a liquid environment in a region spaced apart from the photoconductor substrate by a permeation layer;
applying a voltage to at least one conductor, the at least one conductor positioned above the photoconductor substrate in contact with the liquid environment; and
illuminating at least a portion of the photoconductor substrate with a light source. - View Dependent Claims (40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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Specification