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Method for detection of drug-induced mutations in the reverse transcriptase gene

  • US 6,713,251 B2
  • Filed: 08/31/2001
  • Issued: 03/30/2004
  • Est. Priority Date: 01/26/1996
  • Status: Expired due to Fees
First Claim
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1. A kit for inferring the nucleotide sequence at codons of interest in the HIV reverse transciptase (RT) and/or the amino acids corresponding to these codons and/or the HIV RT resistance spectrum of HIV isolates present in a biological sample comprising:

  • (i) optionally, a means for releasing, isolating, or concentrating the polynucleic acids present in said sample;

    (ii) optionally, at least one suitable set of primers;

    (iii) at least two different probes, wherein each probe is capable of hybridizing specifically to one or more target codons within any region I to VIII as represented in FIG. 1, said probes optionally fixed to a solid support;

    (iv) a hybridization buffer, or components necessary for producing said buffer;

    (v) a wash solution, or components necessary for producing said solution;

    (vi) optionally, a means for detecting the hybrids resulting from the preceding hybridization;

    (vii) optionally, a means for attaching said probe to a solid support wherein the set of primers is selected from the group consisting of;

    SEQ ID No;

    162 and 163, and SEQ ID No;

    164 and 39.

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