Analytical method and apparatus

• US 6,714,303 B2
• Filed: 09/16/2002
• Issued: 03/30/2004
• Est. Priority Date: 02/04/1997
• Status: Expired due to Term

• Current Assignee: GE Healthcare Bio-Sciences AB (GE Healthcare Technologies, Inc.)
• Original Assignee: Biacore AB (General Electric Company)
• Inventors: Ivarsson, Bengt
• Primary Examiner(s): Font, Frank G.
• Assistant Examiner(s): MERLINO, AMANDA H
• Application Number:
  US10/244,819
• Publication Number:
  US 20030067612A1
• Time in Patent Office: 561 Days
• Field of Search: 356/445, 356/446-448, 356/630-632
• US Class Current: 356/445
• CPC Class Codes: G01B 11/0625 with measurement of absorption or reflection
• CPC Class Codes: G01N 2021/212 Arrangement with total internal reflection
• CPC Class Codes: G01N 2021/215 Brewster incidence arrangement
• CPC Class Codes: G01N 21/552 Attenuated total reflection
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658

Abstract

A method of examining thin layer structures on a surface for differences in respect of optical thickness, which method comprises the steps of: irradiating the surface with light so that the light is internally or externally reflected at the surface; imaging the reflected light on a first two-dimensional detector; sequentially or continuously scanning the incident angle and/or wavelength of the light over an angular and/or wavelength range; measuring the intensities of light reflected from different parts of the surface and impinging on different parts of the detector, at at least a number of incident angles and/or wavelengths, the intensity of light reflected from each part of the surface for each angle and/or wavelength depending on the optical thickness of the thin layer structure thereon; and determining from the detected light intensities at the different light incident angles and/or wavelengths an optical thickness image of the thin layer structures on the surface. According to the invention, part of the light reflected at said surface is detected on a second detector to determine the incident angle or wavelength of the polarized light irradiating the surface. An apparatus for carrying out the method is also disclosed.

Claims

1. A method of determining interaction of at least one species with a plurality of individual zones of a sensing surface, which method comprises:

contacting the sensing surface with a fluid sample containing at least one surface interacting species,

irradiating the surface with light so that the light is internally or externally reflected at the surface,

imaging reflected light on a first photo-detector, each individual sensing surface zone corresponding to a respective area of the detector,

sequentially or continuously scanning the incident angle at the sensing surface and/or the wavelength of the light over an angular and/or wavelength range,

measuring the intensities of light imaged on different areas of the detector, at at least a number of incident angles and/or wavelengths to create a series of images of the sensing surface,

simultaneously measuring the momentary incident angle and/or wavelength of the scanned light on a second photo-detector to correlate each image to a specific incident angle and/or wavelength, and

determining from the relationship between image intensity data and angular and/or wavelength data, interaction of the species with the individual sensing surface zones.

2. The method according to claim 1, wherein the incident angle and/or wavelength and the image area intensities of light are measured on a common detector.

3. The method according to claim 1, wherein the incident angle of the light is scanned.

4. The method according to claim 1, wherein the wavelength of the light is scanned.

5. The method according to claim 1, wherein the light is internally reflected from the surface.

6. The method according to claim 1, wherein the light is externally reflected from the surface.

7. The method according to claim 1, wherein the light is coupled to the surface via a prism.

8. The method according to claim 1, wherein the light is coupled to the surface via a grating.

9. The method according to claim 1, wherein the image forming is based on surface plasmon resonance.

10. The method according to claim 1, wherein the determined interaction is a chemical or biochemical binding interaction.

11. The method according to claim 1, wherein the determined interaction is a physical or biophysical interaction.

12. The method according to claim 1, wherein the fluid sample is contacted with the sensing surface in a flow cell.

13. The method according to claim 1, which comprises reading image intensity data into an image data memory and reading angular and/or wavelength data into an angular data memory and/or a wavelength data memory.

14. An analytical system, comprising:

a sensor unit having a sensing surface with a number of individual zones,

means for illuminating the sensing surface with a collimated beam of light,

means for imaging reflected light from the illuminated sensing surface into an image plane,

means for sequentially or continuously scanning the light incident at the sensing surface over a range of incident angles and/or wavelengths,

means for synchronized optical detection of images in the image plane and incident angle and/or wavelength of light illuminating the sensing surface, and

evaluation means for determining from the relationship between detected intensity of different parts of the images and incident light angle and/or wavelength, the optical thickness of each zone of the sensing surface.

15. The system according to claim 14, wherein the means for synchronized optical detection comprises integral photodetector means.

16. The system according to claim 14, wherein the means for scanning comprise beam deflecting means to produce an angle-scanned collimated illumination of the sensing surface, each sensing surface zone momentarily being illuminated by light rays of identical angle of incidence and wavelength.

17. The system according to claim 16, wherein the evaluation means comprise an evaluation unit for determining the angle, and/or the wavelength, for minimum reflectance of p-polarized light, and/or the relative reflectance and phase of the p- and s-polarized electric field components of the light for each of the individual zones of the sensing surface.

18. The system according to claim 16, wherein sensing surface supports reactants capable of binding interaction with species in a sample.

19. The system according to claim 14, wherein the system comprises a sample solution container in contact with the sensing surface to expose the sensing surface to sample solution.

20. The system according to claim 19, wherein the sample solution container is a flow cell.

21. The system according to claim 19, wherein the collimated light beam is p-polarized.

22. The system according to claim 14, wherein the system is based on total internal reflection versus angle and/or wavelength of incidence.

23. The system according to claim 22, wherein the system is based on surface plasmon resonance, Brewster angle, ellipsometry, critical angle, or frustrated total reflection waveguide resonance.

24. The system according to claim 14, wherein the system comprises computer means.