Rupestris stem pitting associated virus nucleic acids, proteins, and their uses

• US 6,716,967 B1
• Filed: 05/09/2000
• Issued: 04/06/2004
• Est. Priority Date: 05/20/1997
• Status: Expired due to Fees

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Gonsalves, Dennis, Meng, Baozhong
• Primary Examiner(s): Housel, James
• Assistant Examiner(s): LI, BAO Q
• Application Number:
  US09/568,189
• Time in Patent Office: 1,428 Days
• Field of Search: 436/5, 436/6, 436/91.2, 436/7.1, 536/22.1, 536/24.32, 530/388.1, 530/389.1, 530/391.5, 424/144.1, 424/801, 424/139.1
• US Class Current: 530/388.1
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/8283 for virus resistance
• CPC Class Codes: C12N 2770/26022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12Q 1/701 Specific hybridization probes
• CPC Class Codes: Y10S 424/801 involving antibody or fragment thereof produced by recombinant dna technology

Abstract

This invention relates to purified monoclonal antibodies that specifically bind to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide, and methods of detecting a Rupestris stem pitting associated virus.

Claims

1. A purified monoclonal antibody or binding portion thereof that specifically binds to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide, wherein said coat protein or coat protein polypeptide is selected from the group consisting of:

(a) a coat protein or coat protein polypeptide encoded by the nucleic acid molecule of SEQ ID NO;

10, SEQ ID NO-21, or SEQ ID NO;

32;

(b) a coat protein or coat protein polypeptide having the amino acid sequence of SEQ ID NO;

11;

(c) a coat protein or coat protein polypeptide having the amino acid sequence of SEQ ID NO;

22; and

(d) a coat protein or coat protein polypeptide having the amino acid sequence of SEQ ID NO;

33.

2. The purified monoclonal antibody of claim 1, wherein said monoclonal antibody or binding portion thereof that specifically binds to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide encoded by the nucleic acid molecule of SEQ ID NO:

10.

3. The purified monoclonal antibody of claim 1, wherein said monoclonal antibody or binding portion thereof that specifically binds to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide encoded by the nucleic acid molecule of SEQ ID NO:

21.

4. The purified monoclonal antibody of claim 1, wherein said monoclonal antibody or binding portion thereof that specifically binds to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide encoded by the nucleic acid molecule of SEQ ID NO:

32.

5. The purified monoclonal antibody of claim 1, wherein said monoclonal antibody or binding portion thereof that specifically binds to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide having the amino acid sequence of SEQ ID NO:

11.

6. The purified monoclonal antibody of claim 1, wherein said monoclonal antibody or binding portion thereof that specifically binds to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide having the amino acid sequence of SEQ ID NO:

22.

7. The purified monoclonal antibody of claim 1, wherein said monoclonal antibody or binding portion thereof that specifically binds to a Rupestris stem pitting associated virus coat protein or cost protein polypeptide having the amino acid sequence of SEQ ID NO:

33.

8. The purified monoclonal antibody of claim 5, wherein said monoclonal antibody binding portion thereof that specifically binds to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide that is SEQ ID NO:

11.

9. The purified monoclonal antibody of claim 6, wherein said monoclonal antibody binding portion thereof that specifically binds to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide that is SEQ ID NO:

22.

10. The purified monoclonal antibody of claim 7, wherein said monoclonal antibody or binding portion thereof that specifically binds to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide that is SEQ ID NO:

33.

11. A method for determining whether a Rupestris stem pitting associated virus is present in a sample, said method comprising the steps of:

(a) contacting a sample with the monoclonal antibody or binding portion thereof of claim 1 under conditions that allow binding of the monoclonal antibody or binding portion thereof to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide; and

(b) detecting a binding reaction between the monoclonal antibody or binding portion thereof and the coat protein or coat protein polypeptide, wherein an undetectable binding reaction indicates that Rupestris stem pitting associated virus is not present in the sample.

12. The method of claim 11, further comprising selecting said sample.

13. The method of claim 11, wherein the binding reaction is detected using enzyme-linked immunoabsorbent assay, radioimmunoassay, gel diffusion precipitin reaction assay, immunodiffusion assay, agglutination assay, fluorescent immunoassay, protein A immunoassay, or immunoelectrophoresis assay.

14. The method of claim 11, wherein said sample comprises a plant cell or tissue.

15. The method of claim 11, wherein said sample comprises plant material.

16. The method of claim 15, wherein said plant material comprises grape plant material.

17. The method of claim 16, wherein said grape plant material comprises a rootstock or scion.

18. The method of claim 11, wherein said sample comprises propagated plant material.

19. The method of claim 11, wherein said binding reaction detects RSPaV-1.

20. The method of claim 11, wherein said binding reaction detects RSP47-4.

21. The method of claim 11, wherein said binding reaction detects RSP-158.

22. A method for determining whether a Rupestris stem pitting associated virus is present in a sample, said method comprising the steps of;

(a) contacting a sample with the monoclonal antibody or binding portion thereof of claim 1 under conditions that allow binding of the monoclonal antibody or binding portion thereof to a Rupestris stem pitting associated virus coat protein or coat protein polypeptide; and

(b) detecting a binding reaction between the monoclonal antibody or binding portion thereof and the coat protein or coat protein polypeptide, said binding reaction indicating that Rupestris stem pitting associated virus is present in the sample.

23. The method of claim 22, further comprising selecting said sample.

24. The method of claim 22, wherein the binding reaction is detected using enzyme-linked immunoabsorbent assay, radioimmunoassay, gel diffusion precipitin reaction assay, immunodiffusion assay, agglutination assay, fluorescent immunoassay, protein A immunoassay, or immunoelectrophoresis assay.

25. The method of claim 22, wherein said sample comprises a plant cell or tissue.

26. The method of claim 22, wherein said sample comprises plant material.

27. The method of claim 26, wherein said plant material comprises grape plant material.

28. The method of claim 27, wherein said grape plant material comprises a rootstock or scion.

29. The method of claim 22, wherein said sample comprises propagated plant material.

30. The method of claim 22, wherein said binding reaction detects RSPaV-1.

31. The method of claim 22, wherein said binding reaction detects RSP47-4.

32. The method of claim 22, wherein said binding reaction detects RSP-158.

33. A method of determining whether a plant is infected with a Rupestris stem pitting associated virus, said method comprising the steps of:

(a) providing a sample from said plant, and

(b) contacting the sample with the monoclonal antibody or binding portion thereof of claim 1 under conditions that allow for a binding reaction between the monoclonal antibody or binding portion thereof and a Rupestris stem pitting associated virus coat protein or coat protein polypeptide, wherein an undetectable binding reaction indicates that the plant is not infected with a Rupestris stem pitting associated virus.

34. The method of claim 33, further comprising selecting a plant that is not infected with a Rupestris stem pitting associated virus.

35. The method of claim 33, wherein the binding reaction is detected using enzyme-linked immunoabsorbent assay, radioimmunoassay, gel diffusion precipitin reaction assay, immunodiffusion assay, agglutination assay, fluorescent immunoassay, protein A immunoassay, or immunoelectrophoresis assay.

36. The method of claim 33, wherein said sample comprises a Plant cell or tissue.

37. The method of claim 33, wherein said sample comprises plant material.

38. The method of claim 37, wherein said plant material comprises grape plant material.

39. The method or claim 38, wherein said grape plant material comprises a rootstock or scion.

40. The method of claim 33, wherein said sample comprises propagated plant material.