Fe(o)-based bioremediation of aquifers contaminated with mixed wastes

• US 6,719,902 B1
• Filed: 08/27/2001
• Issued: 04/13/2004
• Est. Priority Date: 04/25/1997
• Status: Expired due to Fees

• Current Assignee: University of Iowa Research Foundation
• Original Assignee: University of Iowa Research Foundation
• Inventors: Schnoor, Jerald L., Alvarez, Pedro J., Parkin, Gene F., Weathers, Lenly J, Till, Brian A.
• Primary Examiner(s): Prince, Fred G.
• Application Number:
  US09/446,581
• Time in Patent Office: 960 Days
• Field of Search: 210/601, 210/170, 210/615-617, 210/631, 210/747, 210/150, 210/151, 210/903, 210/909, 210/912-914, 435/262, 435/262.5, 435/176, 435/177, 435/180, 405/128.15, 405/128.45
• US Class Current: 210/601
• CPC Class Codes: B09C 1/002 involving in-situ ground water treatment
• CPC Class Codes: B09C 1/10 microbiologically, biologically or by using enzymes
• CPC Class Codes: C02F 3/10 Packings; Fillings; Grids packing elements in general B01J19/30, B01J19/32
• CPC Class Codes: C02F 3/1263 Sequencing batch reactors [SBR]
• CPC Class Codes: C02F 3/286 including two or more steps
• CPC Class Codes: C02F 3/34 characterised by the microorganisms used
• CPC Class Codes: C02F 3/346 Iron bacteria
• CPC Class Codes: Y02W 10/10 Biological treatment of water, waste water, or sewage
• CPC Class Codes: Y10S 210/903 Nitrogenous
• CPC Class Codes: Y10S 210/909 Aromatic compound, e.g. pcb, phenol
• CPC Class Codes: Y10S 210/913 Chromium
• CPC Class Codes: Y10S 210/914 Mercury

Abstract

Disclosed are methods, devices and apparatus for bioremediation of mixed waste aquifers, based on a synergistic combination of reductive treatment using zero-valent iron and anaerobic biotransformations. Also disclosed are methods for in situ and ex situ remediation of groundwater and wastewater via these iron-bacterial compositions in a variety of devices including batch reactors, permeable and semipermeable reactive barriers, flow-through reactors, fluidized bed reactors, and sediment tanks.

Claims

1. A device comprising a composition comprising one or more autotrophic hydrogenotrophic bacteria in culture medium comprising zero-valent iron, wherein said device is comprised within a water-, wastewater- or sewage-treatment system or a system for remediating pollution in an aqueous solution or an environmental site.

2. The device in accordance with claim 1, wherein said hydrogenotrophic bacteria comprise one or more species of bacteria selected from the group consisting of Acetobacterium spp., Achromobacter spp., Aeromonas spp., Acinetobacter spp., Aureobacterium spp., Bacillus spp., Comamonas spp., Dehalobacter spp., Dehalospirillum spp., Dehalococcoide spp., Desulfurosarcina spp., Desulfomonile spp., Desulfobacterium spp., Enterobacter spp., Hydrogenobacter spp., Methanosarcina spp., Pseudomonas spp., Shewanella spp., Methanosarcina spp., Micrococcus spp., and Paracoccus spp.

3. The device of claim 2, wherein said hydrogenotrophic bacteria comprise one or more strains of bacteria selected from the group consisting of Acetobacterium woodi, Aeromonas hydrophila, Aeromonas sobria, Alcaligenes eutrophus, Comamonas acidovorans, Dehalococcoide restrictus, Dehalococcoide multivorans, Dehalococcoide ethenogene, Desulfobacterium tiedje, Enterobacter agglomerans, Hydrogenobacter thermophilus, Methanosarcina barkeri, Methanosarcina mazei, Methanosarcina thermophila, Paracoccus denitrificans, Pseudomonas aureofaciens, Pseudomonas maltophilia, Pseudomonas mendocina, and Shewanella putrefaciens.

4. The device of claim 3, wherein said hydrogenotrophic bacteria comprise Paracoccus denitrifcans ATCC17741, Paracoccus denitrificans ATCC35512, Paracoccus denitrificans ATCC 13543, or Paracoccus denitrificans ATCC 19367.

5. The device of claim 1, wherein said zero-valent iron comprises Fe(0) metal, an Fe(0) alloy, or an Fe(0)—

Ni(0), Fe(0)—

Zn(0), Fe(0)—

Pt(0), or Fe(0)—

Pd(0) bimetal.

6. The device of claim 5, wherein said zero-valent iron comprises filings, shavings, turnings, wool, powder, mesh, beads, rods, pellets, or flakes.

7. The device of claim 1, further comprising a support.

8. The device of claim 7, further comprising a glass, concrete, metallic, zeolite, mineral, fiber, fiberglass, ceramic, plastic, polymeric, or resin support.

9. The device of claim 1, further defined as an ex situ bioreactor.

10. The device in accordance with claim 9, comprising an inlet port, an outlet port and a container means for containing said composition.

11. The device of claim 10, further defined as a continuous culture system, a flow-through packed column, an inline water filter, a biofermenter, a fluidized bed, a sequencing batch reactor, or an anaerobic digester.

12. The device of claim 1, comprised within a water-, wastewater- or sewage-treatment system.

13. The device in accordance with claim 12, comprised within a water treatment system, a sewage or wastewater treatment system, a municipal water supply system, or a pollution decontamination system.

14. The device of claim 1, comprised within a system for remediating pollution in an aqueous solution or an environmental site.

15. A device comprising a composition comprising one or more autotrophic hydrogenotrophic bacteria in culture medium comprising zero-valent iron, said device being comprised within an environmental site.

16. The device of claim 15, comprised within a landfill site, an agricultural site, an agricultural runoff site, or an irrigation site.

17. The device of claim 15, further defined as an in situ reactive barrier.

18. The device in accordance with claim 17, further defined as a permeable barrier, a semipermeable barrier, a treatment wall, and injected treatment zone, or a funnel and gate system.

19. A method of removing or reducing the concentration of an organic or inorganic compound in an environmental site, comprising providing to said site an effective amount of a composition comprising one or more hydrogenotrophic bacteria and zero-valent iron, or contacting said site with a device comprising a composition comprising culture medium comprising one or more hydrogenotrophic bacteria and zero-valent iron.

20. A method for denitrifying groundwater or an environmental site in situ comprising contacting said groundwater or said environmental site with a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria, or contacting said site with a device comprising a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria.

21. A method for removing or reducing the concentration of a nitrogen- or sulfur-containing compound in a sample, comprising contacting a sample suspected of containing said compound with a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria, or contacting said site with a device comprising a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria.

22. The method in accordance with claim 21, wherein said sulfur-containing compound is sulfate or sulfite.

23. A method for removing or reducing the concentration of a halocarbon compound in a sample, comprising contacting a sample suspected of containing said halocarbon with a composition comprising one or more autotrophic hydrogenotrophic bacteria and zero-valent iron, or contacting said sample with a device comprising a composition comprising culture medium comprising one or more autotrophic hydrogenotrophic bacteria and zero-valent iron.

24. The method in accordance with claim 23, wherein said halocarbon is carbon tetrachloride, dichloromethane, a polychlorinated biphenyl, a chlorinated benzene, trichloroethylene, perchloroethylene, dichloroethylene, vinyl chloride, chloroethane, bromoform, dichlorodifluoromethane, trihalomethanes, tetrachlorodibenzodioxin pentachlorophenol, a chlorobenzoate, atrazine, or 1,1,1-TCA.

25. The method of claim 24, wherein said halocarbon is carbon tetrachloride, dichloromethane, trichloroethylene, perchloroethylene, dichloroethylene, vinyl chloride, chloroethane, dichlorodifluoromethane, trihalomethanes, tetrachlorodiberzodioxin pentachlorophenol, a chlorobenzoate, atrazine, or 1,1,1-TCA.

26. The method of claim 25, wherein said halocarbon is carbon tetrachloride, trichloroethylene, or dichloromethane.

27. A method for removing or reducing the concentration of a haloaromatic compound in a sample, comprising contacting a sample suspected of containing said haloaromatic compound with a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria, or contacting said sample with a device comprising a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria.

28. The method in accordance with claim 27, wherein said haloaromatic compound is a polychlorinated biphenyl, a chlorinated benzene, tetrachlorodibenzodioxin pentachlorophenol, a chlorobenzoate, atrazine, or 1,1,1-TCA.

29. A method for degrading or detoxifying a pesticide, comprising contacting a sample suspected of containing said pesticide with a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria, or contacting said sample with a device comprising a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria.

30. The method in accordance with claim 29, wherein said pesticide is methoxyclor, alachlor, metolachlor, lindane, DDT, DDE, DDD, dieldrin, aldrin, heptachlor, chlordane, 2,4-dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid or atrazine.

31. The method of claim 30, wherein said pesticide is atrazine.

32. A method for detoxifying a metal ion-containing compound, comprising contacting a sample suspected of containing said compound with a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria, or contacting said sample with a device comprising a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria.

33. The method in accordance with claim 32, wherein said compound comprises strontium (II), cesium (I), chromium (VI) uranium (VI), technetium (VII), silver (I), or mercury (II).

34. The method of claim 33, wherein said compound comprises chromium (VI) or uranium (VI).

35. A method for reducing the concentration of nitrite-, nitrate-, sulfite-, or sulfate-containing compound in an aqueous solution or environmental site, comprising (a) selecting an aqueous solution or an environmental site containing said compound;

and (b) contacting said solution or site with a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria, or contacting said solution or site with a device comprising a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria.

36. A method for reducing the concentration of a pesticide or organic pollutant in an aqueous solution or environmental site, comprising (a) selecting an aqueous solution or an environmental site containing said pesticide or pollutant;

and (b) contacting said solution or site with a composition comprising zero-valent iron and a culture of one or more autotrophic hydrogenotrophic bacteria, or contacting said solution or site with a device comprising a composition comprising zero-valent iron and a culture of one or more autotrophic hydrogenotrophic bacteria.

37. A method for reducing the concentration of a mercury-, silver-, technetium-, strontium-, cesium-, chromium- or uranium-containing pollutant in an aqueous solution or environmental site, comprising (a) selecting an aqueous solution or an environmental site containing said pollutant;

and (b) contacting said solution or site with a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria, or contacting said solution or site with a device comprising a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria.

38. A method for reducing silver (I), mercury (II), technetium (VII), strontium (II), cesium (I), chromium (VI) or uranium (VI) ions in an aqueous solution, comprising contacting an aqueous solution suspected of containing one or more of said ions with a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria, or contacting said aqueous solution with a device comprising a composition comprising zero-valent iron and a culture of one or more hydrogenotrophic bacteria.

39. A method for removing or reducing the concentration of a nitroaromatic compound in a sample comprising contacting a sample suspected of containing said nitroaromatic compound with one or more hydrogenotrophic bacteria and zero-valent iron, or a device comprising culture medium comprising zero-valent iron.

40. The method in accordance with claim 39, wherein said nitroaromatic compound is trinitrotoluene, RDX, HMX, 2-aminodintrotoluene, 4-aminodinitrotoluene, or parathion.

41. The method in accordance with claim 39, wherein said nitroaromatic compound is trinitrotoluene, RDX, or HMX.