Genetic assay system

• US 6,720,143 B2
• Filed: 04/27/2001
• Issued: 04/13/2004
• Est. Priority Date: 05/27/1999
• Status: Expired due to Term

• Current Assignee: Orchid Cellmark Incorporated (Laboratory Corporation Of America Holdings)
• Original Assignee: Orchid Biosciences Incorporated (Laboratory Corporation Of America Holdings)
• Inventors: Dapprich, Johannes, Scribner, Richard, Juncosa, Robert D., Bongard, Rene
• Primary Examiner(s): FORMAN, BETTY J
• Application Number:
  US09/844,980
• Publication Number:
  US 20010051113A1
• Time in Patent Office: 1,082 Days
• Field of Search: 435/288.5, 435/288.3, 435/287.2, 435/6, 422/50, 422/58, 422/68.1
• US Class Current: 435/6.11
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/50855 using modular assemblies of strips or of individual wells

Abstract

A genetic analysis device particularly for determining the presence or absence of Single Nucleotide Polymorphisms (SNPs) within specific sequences of DNA. The device includes a housing, at least one glass slide member, and an elastomeric member with channels thereon. Oligo arrays are spotted on the glass slide member(s) and subjected to DNA samples, reagents or the like. A plurality of openings or ports allow entry of samples, reagents or wash materials, while a plurality of exit ports or openings allow removal of such materials. The assay devices can be used for multiple samples or a single sample. A plurality of synthesis devices can be positioned in a support base in order to allow sampling in an automated manner. The synthesis devices can be provided in a 96 well microtiter format.

Claims

1. A analysis device comprising:

a housing;

at least one glass slide member positioned in the housing;

an elastomer member positioned in said housing and said housing urging said elastomer member into sealing arrangement with said at least one glass slide member, said elastomer member having at least one channel thereon, at least one inlet port and at least one outlet port;

wherein materials entering said housing through said at least one inlet port are transported through said at least one channel and out through said at least one outlet port.

2. The analysis device as claimed in claim 1 wherein a plurality of inlet ports and a plurality of outlet ports are provided in said elastomer member.

3. The analysis device as claimed in claim 1 wherein two glass slide members are provided, one positioned on each side of said elastomer member, and wherein said elastomer member has at least one channel on each side.

4. The analysis device as claimed in claim 1 wherein said elastomer member provides a liquid tight seal on said glass slide member without the need for adhesives, gaskets or other sealing members between the glass slide member and the elastomer member.

5. The analysis device as claimed in claim 4 wherein said elastomer member is made from a material selected from the group comprising polydimethylsiloxane (PDMS), liquid silicone rubber (LSR) or other elastomeric material having an inherent sealing affinity.

6. A system for analyzing materials comprising:

at least one analysis device and a support base,

(a) said analysis device comprising;

(i) a housing;

(ii) at least one glass slide member positioned in the housing;

(iii) an elastomer member within said housing, said housing urging said elastomer member into a sealing arrangement with said at least one glass slide member, said elastomer member having at least one channel thereon, at least one inlet port and at least one outlet port;

(iv) wherein materials entering through said at least one inlet port are transported through said at least one channel and out through said at least one outlet port, and

(b) said support base comprising a housing having a control portion and a recess, said recess having space for a plurality of analysis devices, and said control portion having a mechanism for eliminating waste materials ejected from said analysis devices.

7. The system as claimed in claim 6 further comprising evaluation means for inspecting said at least one slide member.

8. A device comprising:

a housing having a first portion and a second portion, said first portion engaging said second portion;

at least one glass slide member positioned between the first housing portion and the second housing portion;

an elastomer member positioned between said first housing portion and said second housing portion so that when assembled said first housing portion and said second housing portion urge said elastomer member into a sealing arrangement with said at least glass slide member, said elastomer member having at least one channel, at least one inlet port and at least one outlet port and a material area;

wherein materials entering said housing though said at least one inlet port are transported through said at least one channel and out though said at least one outlet port.

9. The synthesis device as recited in claim 8 further comprising a material site affixed on said slide within said assay area.

10. The device as recited in claim 9 further comprising a window through said first housing portion adjacent to said material sight so that analysis of the material site may be performed therethrough.

11. The device as claimed in claim 8 wherein a plurality of inlet ports and a plurality of outlet ports are provided in said elastomer member.

12. The device as claimed in claim 8 wherein two glass slide members are provided, one positioned side of said elastomer member, and wherein said elastomer member has at least one channel on each side.

13. The device as claimed in claim 8 wherein said elastomer member provides a liquid tight seal on said glass slide member without the need for adhesives, gaskets or other sealing members between the glass slide member and the elastomer member.

14. The device as claimed in claim 8 wherein said elastomer member is made from a material selected from the group comprising polydimethylsiloxane (PDMS), liquid silicone rubber (LSR) or other elastomeric material having an inherent sealing affinity.