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Method and compositions for ordering restriction fragments

  • US 6,720,179 B1
  • Filed: 04/14/2000
  • Issued: 04/13/2004
  • Est. Priority Date: 02/23/1998
  • Status: Expired due to Term
First Claim
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1. A vector containing a restriction fragment of DNA, the vector having a top strand and a bottom strand, and comprising, in sequence:

  • a first type IIs recognition site oriented in the 5′

    to 3′

    direction of said top strand, said restriction fragment, and a second type IIs recognition site oriented in the 5′

    to 3′

    direction of said bottom strand, such that a first type IIs restriction endonuclease recognizing the first type IIs recognition site has a cleavage site which is 3′

    of the first recognition site in the top strand 5′

    to 3′

    direction, and is within the restriction fragment, and a second type IIs restriction endonuclease recognizing the second type IIs recognition site has a cleavage site which is 3′

    of the second recognition site in the bottom strand 5′

    to 3′

    direction, and is within the restriction fragment.

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