Microscope and method for analyzing acquired scan data

• US 6,721,690 B2
• Filed: 12/10/2000
• Issued: 04/13/2004
• Est. Priority Date: 12/10/1999
• Status: Active Grant

• Current Assignee: Leica Microsystems Gmbh (Danaher Corporation)
• Original Assignee: Leica Microsystems Gmbh (Danaher Corporation)
• Inventors: Olschewski, Frank
• Primary Examiner(s): Hoff, Marc S.
• Assistant Examiner(s): SUAREZ, FELIX E
• Application Number:
  US09/681,051
• Publication Number:
  US 20020027193A1
• Time in Patent Office: 1,220 Days
• Field of Search: 702/57, 702/66, 702/67, 702/69, 702/71-74, 702/93, 702/111, 702/124, 702/181, 702/183, 702/188, 702/189, 356/456, 600/407
• US Class Current: 702/189
• CPC Class Codes: G02B 21/002 Scanning microscopes scanning near field optical microscopes G01Q60/18
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence

Abstract

A method and system for analyzing acquired scan data are disclosed. The method involves measuring physical values of a particular process, converting the measured values into two signals, such as electrical signals, and then converting the two signals into polar coordinates by defining a vector having a radial magnitude and a vector angle corresponding to the magnitudes of the two signals. The method further provides for validation and visual representation of the measured physical values by employing the angle information to define the upper and lower bounds of the acceptable measured values. The corresponding system comprises sensors measuring the two physical values and yielding the two signals. A signal processing unit receives the two signals, converts them into a pair of polar coordinates defines by the magnitude of the two signals.

Claims

1. A method for signal processing, the method comprising the steps of:

measuring first and second physical values relating to a phenomenon of interest in a confocal microscope, yielding first and second signals indicative thereof, each of the first and second signals containing noise;

converting the first and second signals into polar coordinates indicative of a vector defined by the magnitudes of the first and second signals, the polar coordinates defining radial magnitude and vector angle relative to a coordinate system; and

employing upper and lower bounds of the angle information, and upper bounds of the first and second signals, to validate the measured values.

2. The method of claim 1 wherein the vector is defined by the magnitudes of the first and second signals expressed along orthogonal axes.

3. The method of claim 1 wherein the first and second values are measured intensities at differing optical wavelengths, the intensities measured by means of a scanning flourescence microscope.

4. The method of claim 1 wherein the conversion is performed by at least one field programmable gate array.

5. The method of claim 1 wherein the conversion is performed by at least one digital signal processor.

6. The method of claim 1 wherein the conversion is performed by a general microcomputer.

7. The method of claim 1 wherein the conversion is performed with the use of a two lookup table.

8. The method of claim 1 wherein the conversion is performed with the use of a COORDIC algorithm.

9. The method of claim 1 wherein the conversion is performed by means of direct calculation using division, adders and multipliers and lookup tables.

10. The method of claim 1 wherein the conversion is performed by means of approximation via a digital neural network.

11. The method of claim 1 wherein the first and second values are measured intensities at differing optical wavelengths, the intensities indicative of Ca 2+ concentrations.

12. The method of claim 1 wherein the first and second values are measured intensities at differing optical wavelengths, the intensities indicative of pH.

13. The method of claim 1 wherein the first and second values are measured intensities at differing optical wavelengths, the intensities indicative of cAMP.

14. The method of claim 1 wherein the first arid second values are measured intensities at differing optical wavelengths, the intensities measured by means of a cytofluorimeter.

15. A signal processing system comprising:

sensors measuring first and second physical values relating to a phenomenon of interest in a confocal microscope, yielding first and second signals indicative of thereof;

signal processing apparatus receiving the first and second signals and converting the first and second signals into polar coordinates indicative of a vector defined by the magnitudes of the first and second signals.

16. The system of claim 15 wherein the first an second signal are electrical signals.

17. The system of claim 15 wherein the first and second values are measured intensities at differing optical wavelengths, the intensities measured by means of a scanning flourescence microscope.

18. A method for validating measurements of physiological phenomena, the method comprising the steps of:

measuring first and second physical values relating to a phenomenon of interest, yielding first and second signals indicative thereof, each of the first and second signals containing noise;

converting the first and second signals into polar coordinates indicative of a vector defined by the magnitudes of the first and second signals, the polar coordinates defining radial magnitude and vector angle relative to a coordinate system;

applying a first threshold comparator to the first signal, the comparator defining a first in-region signal when the first signal is below a first predetermined threshold;

applying a second threshold comparator to the second signal, the comparator defining a second in-region when the second signal is below a second predetermined threshold;

applying a third threshold comparator to the angle signal, the comparator defining a third in-region signal when the angle signal is below a third predetermined threshold;

applying a fourth threshold comparator to the angle signal, the comparator defining a fourth in-region signal when the first signal is above a predetermined threshold;

annunciating the event of the presence of the first, second, and fourth in-region signals.

19. The method of claim 18 wherein the annunciation is presented to a user in visual form.