Comparative phenotype analysis
First Claim
1. A method for comparing at least two cell preparations, comprising the steps of:
- a) providing a testing device comprising a plurality of testing wells and at least two test substrates selected from the group consisting of phosphorus sources selected from the group consisting of phosphorus-containing compounds, sulfur sources selected from the group consisting of sulfur-containing compounds, and auxotrophic supplements selected from the group consisting of amino acids, vitamins, polyamines, lactones, fatty acids, nucleic acid bases, nucleosides, and nucleotides, wherein each well comprises at least one of said test substrates;
b) preparing a first cell preparation and a second cell preparation, wherein said first and said second cell preparations are each in an aqueous solution;
c) introducing said first and second cell preparations into said testing wells of said testing device;
d) detecting the response of said first and second cell preparations to said at least two test substrates using a single colorimetric indicator; and
e) comparing the response of said first and second cell preparations.
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Abstract
The present invention relates to growing and testing microorganisms in a multitest format which utilizes a gel forming matrix for the rapid screening of clinical and environmental cultures. The present invention is suited for the characterization of commonly encountered microorganisms (e.g., E. coli, S. aureus, etc.), as well as commercially and industrially important organisms from various and diverse environments (e.g., the present invention is particularly suited for the growth and characterization of the actinomycetes and fungi). The present invention is also particularly suited for comparative analysis of phenotypic differences between cell types, including strains of microorganisms that have been designated as the same genus and species, as well as other cell types (e.g., mammalian, insect, and plant cells).
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Citations
55 Claims
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1. A method for comparing at least two cell preparations, comprising the steps of:
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a) providing a testing device comprising a plurality of testing wells and at least two test substrates selected from the group consisting of phosphorus sources selected from the group consisting of phosphorus-containing compounds, sulfur sources selected from the group consisting of sulfur-containing compounds, and auxotrophic supplements selected from the group consisting of amino acids, vitamins, polyamines, lactones, fatty acids, nucleic acid bases, nucleosides, and nucleotides, wherein each well comprises at least one of said test substrates;
b) preparing a first cell preparation and a second cell preparation, wherein said first and said second cell preparations are each in an aqueous solution;
c) introducing said first and second cell preparations into said testing wells of said testing device;
d) detecting the response of said first and second cell preparations to said at least two test substrates using a single colorimetric indicator; and
e) comparing the response of said first and second cell preparations. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A method for comparing at least two cell preparations, comprising the steps of:
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a) providing a testing device comprising a plurality of testing wells and at least two test substrates selected from the group consisting of nitrogen sources selected from the group consisting of nitrogen-containing compounds, phosphorus sources selected from the group consisting of phosphorus-containing compounds, sulfur sources selected from the group consisting of sulfur-containing compounds, and auxotrophic supplements selected from the group consisting of amino acids, vitamins, polyamines, lactones, fatty acids, nucleic acid bases, nucleosides, and nucleotides, wherein each well comprises at least one test substrate;
b) preparing a first cell preparation and a second cell preparation, wherein said first and said second cell preparations are each in an aqueous solution and said aqueous solution comprises at least one carbon source;
c) introducing said first and second cell preparations into said testing wells of said testing device;
d) detecting the response of said first and second cell preparations to said at least two test substrates using a single colorimetric indicator; and
e) comparing the response of said first and second cell preparations. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55)
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Specification