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Method of monitoring the temperature of a biochemical reaction

  • US 6,730,478 B1
  • Filed: 03/06/2001
  • Issued: 05/04/2004
  • Est. Priority Date: 09/07/1998
  • Status: Expired due to Fees
First Claim
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1. A method of monitoring the temperature of a biochemical reaction in a reaction mixture, said method comprising effecting the reaction in the presence of a fluorescently labelled temperature probe DNA sequence which comprises a double stranded region which denatures at a predetermined temperature, the fluorescent label of said temperature probe sequence being arranged so that a detectable signal occurs at the point at which denaturation of said region takes place;

  • and monitoring fluorescence from said reaction mixture so as to determine when said predetermined temperature has been reached, wherein the temperature probe comprises a single nucleic acid strand, end regions of which hybridise together so as to form a loop or hairpin structure and wherein the fluorescent label comprises an intercalating dye.

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