Total analyte quantitation
First Claim
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1. A system for separating two or more analytes and determining a total analyte concentration or amount, the system comprising:
- (i) a microfluidic device comprising a body structure having a plurality of microscale channels disposed therein, the microscale channels comprising;
(a) a first channel region;
(b) a measurement channel region fluidly coupled to the first channel region;
(c) a separation channel fluidly coupled to the first channel region;
(d) a first detection region, which first detection region is positioned proximal to the measurement channel region; and
, (e) a second detection region, which second detection region is positioned proximal to the separation channel;
(ii) a fluid direction system fluidly coupled or air coupled to the microfluidic device, which fluid direction system directs;
(a) movement of at least two analytes through the first channel region;
(b) movement of the at least two analytes from the first channel region into the measurement channel region;
(c) movement of the at least two analytes through the first detection region;
(d) movement of the at least two analytes from the first channel into the separation channel, thereby producing two or more separated analytes; and
, (e) movement of the two or more separated analytes through the second detection region; and
, (iii) a detection system positioned proximal to one or more of;
the first detection region or the second detection region, which detection system is configured to detect the at least two analytes in the first detection region, producing a total analyte signal, which total analyte signal corresponds to the total analyte concentration or amount, and detects the two or more separated analytes in the second detection region, producing two or more analyte signals, which two or more analyte signals correspond to the two or more separated analytes.
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Abstract
Methods for determining total analyte concentrations and amounts, especially in combination with analyte separations are provided. Microfluidic devices are used to separate analyte mixtures and detect the individual analytes. Signal areas are summed for each individual analyte to quantitate the total analyte amount. Separate measurements of the total analyte sample are also used to determine total analyte concentration.
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Citations
23 Claims
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1. A system for separating two or more analytes and determining a total analyte concentration or amount, the system comprising:
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(i) a microfluidic device comprising a body structure having a plurality of microscale channels disposed therein, the microscale channels comprising;
(a) a first channel region;
(b) a measurement channel region fluidly coupled to the first channel region;
(c) a separation channel fluidly coupled to the first channel region;
(d) a first detection region, which first detection region is positioned proximal to the measurement channel region; and
,(e) a second detection region, which second detection region is positioned proximal to the separation channel;
(ii) a fluid direction system fluidly coupled or air coupled to the microfluidic device, which fluid direction system directs;
(a) movement of at least two analytes through the first channel region;
(b) movement of the at least two analytes from the first channel region into the measurement channel region;
(c) movement of the at least two analytes through the first detection region;
(d) movement of the at least two analytes from the first channel into the separation channel, thereby producing two or more separated analytes; and
,(e) movement of the two or more separated analytes through the second detection region; and
,(iii) a detection system positioned proximal to one or more of;
the first detection region or the second detection region, which detection system is configured to detect the at least two analytes in the first detection region, producing a total analyte signal, which total analyte signal corresponds to the total analyte concentration or amount, and detects the two or more separated analytes in the second detection region, producing two or more analyte signals, which two or more analyte signals correspond to the two or more separated analytes.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of introducing a representative aliquot of a sample comprising at least two analytes into a separation channel of a microfluidic device, the method comprising
flowing the sample through a first channel of a microfluidic device, the first channel being in fluid communication with the separation channel at an intersection, while flowing the sample through a detection region, monitoring a signal detected at the detection region, and injecting a sample aliquot from the first channel into the separation channel when the signal indicates that all analytes in the sample have reached the intersection.
Specification