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Total analyte quantitation

  • US 6,733,645 B1
  • Filed: 04/12/2001
  • Issued: 05/11/2004
  • Est. Priority Date: 04/18/2000
  • Status: Expired due to Term
First Claim
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1. A system for separating two or more analytes and determining a total analyte concentration or amount, the system comprising:

  • (i) a microfluidic device comprising a body structure having a plurality of microscale channels disposed therein, the microscale channels comprising;

    (a) a first channel region;

    (b) a measurement channel region fluidly coupled to the first channel region;

    (c) a separation channel fluidly coupled to the first channel region;

    (d) a first detection region, which first detection region is positioned proximal to the measurement channel region; and

    , (e) a second detection region, which second detection region is positioned proximal to the separation channel;

    (ii) a fluid direction system fluidly coupled or air coupled to the microfluidic device, which fluid direction system directs;

    (a) movement of at least two analytes through the first channel region;

    (b) movement of the at least two analytes from the first channel region into the measurement channel region;

    (c) movement of the at least two analytes through the first detection region;

    (d) movement of the at least two analytes from the first channel into the separation channel, thereby producing two or more separated analytes; and

    , (e) movement of the two or more separated analytes through the second detection region; and

    , (iii) a detection system positioned proximal to one or more of;

    the first detection region or the second detection region, which detection system is configured to detect the at least two analytes in the first detection region, producing a total analyte signal, which total analyte signal corresponds to the total analyte concentration or amount, and detects the two or more separated analytes in the second detection region, producing two or more analyte signals, which two or more analyte signals correspond to the two or more separated analytes.

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