Biosensor electrode mediators for regeneration of cofactors and process for using
First Claim
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1. A single use disposable electrode strip for attachment to the signal readout circuitry of a sensor system to detect a current representative of an analyte in an aqueous sample, the strip comprising:
- a) an elongated support having a substantially flat planar surface, adapted for releasable attachment to said readout circuitry;
b) a first conductor extending along said surface and comprising a conductive element for connection to said readout circuitry;
an active electrode on said surface in contact with said first conductor, said active electrode comprising a nicotinamide co-factor-dependent enzyme, a nicotinamide cofactor, and a mediator consisting of a compound having the following formula;
where X and Y are independently oxygen, sulphur, CR3R4, NR3, or NR3R4 or the functional group CZ1Z2, where Z1 and Z2 are electron withdrawing groups; and
R3 and R4 are independently a hydrogen atom, a hydroxyl group or a substituted or unsubstituted alkyl, aryl, heteroaryl, amino, alkoxyl, or aryloxyl group, wherein said active electrode is formulated with filler and binder ingredients;
c) a second conductor extending along said surface, comprising a conductive element for connection to said readout circuitry;
d) a reference/counter electrode in contact with said second conductor;
e) said conductors being spaced apart so as not to be in electrical contact and being configured so as not to be brought into electrical contact when said aqueous sample is placed on said strip;
f) said active electrode and said reference/counter electrode being configured so that both may be simultaneously covered by a small drop of said aqueous sample to provide an electrical conduction path between said electrodes.
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Abstract
The present invention is based on the discovery of NAD+ and NADP+ mediator compounds that do not bind irreversibly to thiol groups in the active sites of intracellular dehydrogenase enzymes. Such mediator compounds avoid a common mode of enzyme inhibition. The mediators can therefore increase the stability and reliability of the electrical response in amperometric electrodes constructed from NAD- or NADP-dependent enzymes.
353 Citations
20 Claims
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1. A single use disposable electrode strip for attachment to the signal readout circuitry of a sensor system to detect a current representative of an analyte in an aqueous sample, the strip comprising:
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a) an elongated support having a substantially flat planar surface, adapted for releasable attachment to said readout circuitry;
b) a first conductor extending along said surface and comprising a conductive element for connection to said readout circuitry;
an active electrode on said surface in contact with said first conductor, said active electrode comprising a nicotinamide co-factor-dependent enzyme, a nicotinamide cofactor, and a mediator consisting of a compound having the following formula;
where X and Y are independently oxygen, sulphur, CR3R4, NR3, or NR3R4 or the functional group CZ1Z2, where Z1 and Z2 are electron withdrawing groups; and
R3 and R4 are independently a hydrogen atom, a hydroxyl group or a substituted or unsubstituted alkyl, aryl, heteroaryl, amino, alkoxyl, or aryloxyl group, wherein said active electrode is formulated with filler and binder ingredients;c) a second conductor extending along said surface, comprising a conductive element for connection to said readout circuitry;
d) a reference/counter electrode in contact with said second conductor;
e) said conductors being spaced apart so as not to be in electrical contact and being configured so as not to be brought into electrical contact when said aqueous sample is placed on said strip;
f) said active electrode and said reference/counter electrode being configured so that both may be simultaneously covered by a small drop of said aqueous sample to provide an electrical conduction path between said electrodes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
a) providing the electrode strip of claim 1;
b) oxidizing the analyte with the NAD(P)+ dependent enzyme in the presence of NAD(P)+;
oxidizing the NAD(P)H generated by reaction with the analyte and NAD(P)+ dependent enzyme with the mediator compound of step a); and
c) applying an electrical potential at an electrode to reoxidize the mediator compound reduced in oxidizing NAD(P)H and observing the resultant current, wherein some of the mediator compound is being reduced by reaction with NAD(P)H while some of the mediator compound is being oxidized by transfer of electrons to said electrode during a measurement period and the rate of oxidation of the mediator compound over said measurement period and consequently the resultant observed current is monotonically related to the concentration of analyte in the sample.
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6. The process of claim 5 wherein the current observed during the measurement period is linearly related to the concentration of the analyte in the sample.
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7. The process of claim 5 wherein the mediator is 1,10-phenanthroline quinone.
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8. The process of claim 5 wherein the co-factor dependent enzyme is glucose dehydrogenase.
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9. The process of claim 5 wherein the co-factor dependent enzyme is hydroxybutyrate dehydrogenase.
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10. The process of claim 5 wherein the applied potential is 200 mV or less.
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11. A single use disposable electrode strip for attachment to the signal readout circuitry of a sensor system to detect a current representative of an analyte in an aqueous sample, the strip comprising:
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a) an elongated support having a substantially flat planar surface, adapted for releasable attachment to said readout circuitry;
b) a first conductor extending along said surface and comprising a conductive element for connection to said readout circuitry;
an active electrode on said surface in contact with said first conductor, said active electrode comprising a nicotinamide co-factor-dependent enzyme, a nicotinamide cofactor, and a mediator consisting of a compound having the following formula;
where X and Y are independently oxygen, sulphur, CR3R4, NR3, or NR3R4 or the functional group CZ1Z2, where Z1 and Z2 are electron withdrawing groups;
R1 and R2 are independently heteroaromatic groups; and
R3 and R4 are independently a hydrogen atom, a hydroxyl group or a substituted or unsubstituted alkyl, aryl, heteroaryl, amino, alkoxyl, or aryloxyl group, wherein said active electrode is formulated with filler and binder ingredients;c) a second conductor extending along said surface, comprising a conductive element for connection to said readout circuitry;
d) a reference/counter electrode in contact with said second conductor;
e) said conductors being spaced apart so as not to be in electrical contact and being configured so as not to be brought into electrical contact when said aqueous sample is placed on said strip;
f) said active electrode and said reference/counter electrode being configured so that both may be simultaneously covered by a small drop of said aqueous sample to provide an electrical conduction path between said electrodes. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
a) providing the electrode strip of claim 11;
b) oxidizing the analyte with the NAD(P)+ dependent enzyme in the presence of NAD(P)+;
oxidizing the NAD(P)H generated by reaction with the analyte and NAD(P)+ dependent enzyme with the mediator compound of step a); and
c) applying an electrical potential at an electrode to reoxidize the mediator compound reduced in oxidizing NAD(P)H and observing the resultant current, wherein some of the mediator compound is being reduced by reaction with NAD(P)H while some of the mediator compound is being oxidized by transfer of electrons to said electrode during a measurement period and the rate of oxidation of the mediator compound over said measurement period and consequently the resultant observed current is monotonically related to the concentration of analyte in the sample.
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17. The process of claim 16 wherein the current observed during the measurement period is linearly related to the concentration of the analyte in the sample.
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18. The process of claim 16 wherein the co-factor dependent enzyme is glucose dehydrogenase.
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19. The process of claim 16 wherein the co-factor dependent enzyme is hydroxybutyrate dehydrogenase.
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20. The process of claim 16 wherein the applied potential is 200 mV or less.
Specification