Precision fluid dispensing system

• US 6,739,478 B2
• Filed: 06/25/2002
• Issued: 05/25/2004
• Est. Priority Date: 06/29/2001
• Status: Expired due to Fees

• Current Assignee: Scientific Products and Systems, University of Maryland
• Original Assignee: Scientific Products and Systems
• Inventors: Anjanappa, Muniswamappa, Song, Tao, Bach, David T., Ragavan, Gayathri S.
• Primary Examiner(s): Bomberg, Kenneth
• Application Number:
  US10/180,710
• Publication Number:
  US 20030000965A1
• Time in Patent Office: 700 Days
• Field of Search: 417/442, 417/485, 417/490, 417/493, 417/498, 417/500, 222/1, 222/136, 222/144.5, 222/309, 222/333
• US Class Current: 222/1
• CPC Class Codes: B01F 25/60 Pump mixers, i.e. mixing within a pump
• CPC Class Codes: B01L 2400/0622 distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
• CPC Class Codes: B01L 3/0206 of the plunger pump type
• CPC Class Codes: B01L 3/0227 Details of motor drive means B01L3/0231, B01L3/0234 take precedence
• CPC Class Codes: F04B 13/00 Pumps specially modified to deliver fixed or variable measured quantities
• CPC Class Codes: F04B 7/04 in which the valving is performed by pistons and cylinders coacting to open and close intake or outlet ports

Abstract

A precision fluid dispensing system containing at least one two-piece pump and a precision closed loop controller drive system to address the small volume precision dispensing requirements of bioscience applications. A multiple diameter pump can be combined with a pump having multiple inlet and outlet ports to allow for precision multiple outlet dispenses in a single pump that finds use with microtiter plate pipetting and other precision dispensing. Inlet ports can be located on the smaller diameter of the cylinder with outlet ports on the larger diameter of the cylinder. A micro-controller with closed loop feedback provides exact linear positioning and motion of the pump piston as well as optional control of a nozzle to provide exact micro-dispensing of fluids.

Claims

1. A precision fluid dispensing system comprising:

a two-piece pump having a two or more diameter piston disposed in an outer cylinder, said outer cylinder having a same number of diameters as said piston, said pump also having a plurality of input and output ports attached to said outer cylinder and defined by said piston and said cylinder;

a fixed frame attached to said outer cylinder, said fixed frame rigidly holding said outer cylinder;

a sliding frame attached to said piston, said sliding frame moving in relation to said fixed frame, said sliding frame displacing said piston by said movement;

a first motor attached to said sliding frame, said first motor coupled to said piston causing said piston to rotate between a plurality of port positions;

a second motor attached to said fixed frame, said second motor causing said sliding frame to move in relation to said fixed frame, whereby said sliding frame displaces said piston;

a closed loop feedback control system with an input and an output, said input proportional to said piston'"'"'s position, said output controlling said second motor, whereby said closed loop feedback control system allows displacement of said piston to precisely dispense a predetermined amount of fluid.

2. The precision fluid dispensing system of claim 1 wherein said two or more diameter piston and cylinder have a smaller and a larger diameter, said inlet ports being located on said smaller diameter.

3. The precision fluid dispensing system of claim 2 wherein said outlet ports are located on said larger diameter.

4. The precision fluid dispensing system of claim 1 wherein said first and second motors are stepper motors.

5. The precision fluid dispensing system of claim 1 wherein only one of said ports is active at a given time.

6. The precision fluid dispensing system of claim 1 wherein at least one input port and at least one outlet port are aligned.

7. The precision fluid dispensing system of claim 1 further comprising a linear scale responsive to the position of said piston, said linear scale providing input to said closed feedback control system.

8. The precision fluid dispensing system of claim 4 wherein said second stepper motor can step at least 125,000 steps per revolution.

9. The precision fluid dispensing system of claim 1 wherein said two piece pump contains an output port coupled to a controllable nozzle.

10. The precision fluid dispensing system of claim 9 wherein said controllable nozzle is directly controlled by said closed loop feedback system.

11. A method of dispensing a predetermined amount of fluid comprising the steps of:

specifying to a closed loop feedback control system a desired amount of fluid to dispense, said closed loop feedback control system coupled to a sliding piston in a two piece pump, said piston rotating between a plurality of inlet and outlet port positions and moving linearly out and in to load and dispense fluid, said closed loop feedback system sensing said piston'"'"'s linear position and controlling said displacement;

causing said piston to rotate to a predetermined inlet port position;

causing said piston to move linearly out thereby loading fluid;

causing said piston to rotate to a predetermined outlet port position;

causing said piston to move linearly in under direct control of said closed loop feedback system thereby dispensing a precise amount of said fluid.

12. The method of claim 11 further comprising high precision position feedback control achieved in two stages.

13. The method of claim 11 wherein said piston is driven by at least one stepper motor.

14. The method of claim 13 wherein said stepper motor can step at least 125,000 steps per revolution.

15. The method of claim 11 wherein said two piece pump is coupled to a controllable nozzle.

16. A system of the type used in biological sciences to dispense precision micro-quantities of fluids, the system comprising, in combination:

a two piece pump means with an outer cylinder containing a plurality of ports with ports and a rotating and sliding inner piston for dispensing fluids;

a processor means for providing closed loop feedback control to said piston, said piston rotatable between port positions and displacable linearly, said processor means controlling a rotational and displacement position of said piston;

linear displacement measurement means for determining the displacement of said piston, said displacement being communicated to said processor means;

motor drive means for causing a linear displacement of said piston, said motor drive means being controlled by said processor means to precisely dispense a predetermined amount of fluid.

17. The system of claim 16 wherein said motor drive means is a stepper motor.

18. The system of claim 17 wherein said stepper motor can step at least 125,000 steps per revolution.

19. The system of claim 16 wherein said stepper motor can step between 500 and 155,000 steps per revolution.

20. The system of claim 16 wherein said two piece pump means is attached to a controllable nozzle.