Photo-induced electron transfer fluorescent sensor molecules
First Claim
1. A method of synthesizing a modular fluorescence sensor comprising the steps of:
- (a) forming an asymmetric compound of the following general formula;
wherein;
Fl is a fluorophore;
N is a nitrogen atom and H is a hydrogen atom;
Sp is an aliphatic spacer;
An is an anchor group for attaching the sensor to a solid substrate; and
n=1 or 2, and x is any integer; and
(b) replacing hydrogen atoms with Bd1 and Bd2 groups, wherein Bd1 and Bd2 are independently selected binding groups capable of binding an analyte molecule to form a stable 1;
1 complex.
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Accused Products
Abstract
Disclosed is a modular fluorescence sensor having the following general formula:
Where Fl is a fluorophore, N is a nitrogen atom, Bd1 and Bd2 are independently selected binding groups, Sp is an aliphatic spacer, and An is an anchor group for attaching the sensor to solid substrates. n=1 or 2, m=1 or 2, x is an integer, and y=1 or 2. The binding groups are capable of binding an analyte molecule to form a stable 1:1 complex. In a preferred embodiment, the Bd1 is R1—B(OH)2 and Bd2 is R2—B(OH)2. R1 and R2 are aliphatic or aromatic functional groups selected independently from each other and B is a boron atom. The present invention also provides methods of synthesizing a modular fluorescence sensor and its use in labeling solid substrates.
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Citations
11 Claims
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1. A method of synthesizing a modular fluorescence sensor comprising the steps of:
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(a) forming an asymmetric compound of the following general formula;
wherein;
Fl is a fluorophore;
N is a nitrogen atom and H is a hydrogen atom;
Sp is an aliphatic spacer;
An is an anchor group for attaching the sensor to a solid substrate; and
n=1 or 2, and x is any integer; and
(b) replacing hydrogen atoms with Bd1 and Bd2 groups, wherein Bd1 and Bd2 are independently selected binding groups capable of binding an analyte molecule to form a stable 1;
1 complex.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification
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- Resources
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Current AssigneeBaxter Healthcare SA (Baxter International Inc.), Baxter International Inc.
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Original AssigneeBeckman Coulter Incorporated (Danaher Corporation)
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InventorsJames, Tony D., Arimori, Susumu
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Primary Examiner(s)Riley, Jezia
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Application NumberUS10/053,274Publication NumberTime in Patent Office859 DaysField of Search252/301.16, 252/301.22, 436/546, 436/94, 436/250, 435/968US Class Current252/301.16CPC Class CodesG01N 33/533 with fluorescent labelY10T 436/143333 Saccharide [e.g., DNA, etc.]
