Bovine VDJ cassette, BF1H1, suitable for antigenization

• US 6,740,747 B2
• Filed: 04/19/2002
• Issued: 05/25/2004
• Est. Priority Date: 04/20/2001
• Status: Expired due to Fees

• Current Assignee: Azad Kaushik, Surinder Singh Saini
• Original Assignee: Azad Kaushik, Surinder Singh Saini
• Inventors: Saini, Surinder Singh, Kaushik, Azad
• Primary Examiner(s): Crouch, Deborah
• Assistant Examiner(s): WOITACH, JOSEPH T
• Application Number:
  US10/125,594
• Publication Number:
  US 20030170646A1
• Time in Patent Office: 767 Days
• Field of Search: 435/320.1, 435/325, 514/44, 424/130.1, 536/23.1, 536/23.4, 536/23.5, 536/23.53
• US Class Current: 536/23.53
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 2317/565 Complementarity determining region [CDR]

Abstract

The present invention relates to a bovine VDJ cassette (BF1H1) that provides the novel ability to develop chimeric immunoglobulin molecule capable of incorporating both linear T cell epitope(s) (CDR1H and CDR2H) as well as conformational B cell epitope(s) (exceptionally long CDR3H). The antigenized immunoglobulin incorporating both T and B epitopes of interest is especially useful for development of oral vaccines for use in humans apart from other species including cattle. The long CDR3H in BF1H1 VDJ rearrangement originates from long germline D-genes. The novel bovine germline D-genes provide additional opportunities for sustaining the capacity for antibody diversification in cattle essential for immunocompetence via selective breeding strategies that incorporate immunoglobulin gene markers. The novel gene elements, such as D-genes, are unique to cattle and, therefore, are useful in forensic analysis.

Claims

1. An isolated bovine VDJ nucleic acid sequence consisting of SEQ ID NO 1.

2. A recombinant plasmid comprising the isolated bovine VDJ nucleic acid sequence according to claim 1.

3. A vector comprising a plasmid according to claim 2.

4. A vector comprising the isolated bovine VDJ nucleic acid sequence according to claim 1.