Methods and compositions to reduce or eliminate transmission of a transgene

• US 6,743,968 B2
• Filed: 02/28/2001
• Issued: 06/01/2004
• Est. Priority Date: 02/28/2000
• Status: Expired due to Term

• Current Assignee: Yale University
• Original Assignee: Yale University
• Inventors: Dellaporta, Stephen L., Moreno, Maria A.
• Primary Examiner(s): Fox, David T.
• Application Number:
  US09/794,384
• Publication Number:
  US 20020144305A1
• Time in Patent Office: 1,189 Days
• Field of Search: 800/271, 800/274, 800/278, 800/287, 800/303, 800/300, 800/288, 800/302, 800/279, 435/199, 435/320.1, 435/419
• US Class Current: 800/274
• CPC Class Codes: C12N 15/8241 Phenotypically and genetically modified plants via recombinant DNA technology
• CPC Class Codes: C12N 15/8289 Male sterility
• CPC Class Codes: C12N 15/829 Female sterility

Abstract

Genetic constructs and methods are disclosed for the production, maintenance and control of transgenes in transgenic eukaryotic organisms that undergo meiosis in which pollen or sperm can be outcrossed; this includes: transgenic animals, plant cells, plant tissues and whole plants. More specifically, this invention relates to the control of transgene transmission by male and/or female gametes or gametophytes using a gametophytic sterility trait (GST). The genetic constructs and methodologies of the present invention provide the ability to control the undesired spread of transgenes. In addition, this invention also provides the tools and methodologies to enrich a plant or other eukaryotic genome for dispersed and/or stable transposition events.

Claims

1. A method of eliminating transmission of a transgene of interest contained within the genome of a plant, wherein the method eliminates the production of pollen containing the transgene of interest by said plant and thereby prevents transmission of the transgene of interest by pollination with another plant, said method comprising:

a) producing a transformed plant that is hemizygotic but not homozygotic or heterozygotic for a nucleic acid construct comprising a pollen-specific promoter operably linked to a suicide coding region in close physical proximity to a transgene of interest;

b) growing the plant in step a) wherein said plant only produces pollen lacking said transgene of interest; and

c) allowing the pollen from the plant grown in step b) to pollinate the same or another plant of the same species;

wherein said pollen does not contain the transgene of interest and transmission of said transgene of interest to said other plant is eliminated.

2. The method of claim 1, wherein said transformed plant produces about 50% less pollen compared to a wild-type non-transformed plant of the same species.

3. The method of claim 1, wherein the construct further comprises a promoter operably linked to a marker suitable for selection of the transformed plants.

4. The method of claim 1, wherein said pollination is cross-pollination.

5. The method of claim 1, wherein said nucleic acid construct comprising a pollen-specific promoter operably linked to a suicide coding region in close physical proximity to a transgene of interest is stably integrated into the genome of said transformed plant.

6. The method of claim 5, wherein said nucleic acid construct comprising the pollen-specific promoter operably linked to the suicide coding region which is in close physical proximity to the transgene of interest, are all maintained in close physical proximity to each other within the genome of the plant after being stably integrated into the genome of said plant.

7. A method of plant pollination that eliminates transmission of a transgene of interest contained within the genome of a plant, wherein the method eliminates the production of pollen containing the transgene of interest by said plant and thereby prevents transmission of the transgene of interest by cross-pollination, said method comprising:

a) producing a transformed plant that is hemizygotic but not homozygotic or heterozygotic for a nucleic acid construct comprising a pollen-specific promoter operably linked to a suicide coding region in close physical proximity to a transgene of interest;

b) growing the plant in step a) wherein said plant only produces pollen lacking said transgene of interest; and

c) allowing the pollen from the plant grown in step b) to cross-pollinate a second plant of the same species;

wherein said pollen does not contain said transgene of interest and transmission of said transgene of interest to said second plant is eliminated.