Disposable plate electrode with biologicalactive film and manufacture method thereof
First Claim
1. A manufacture method for forming a biological active layer on a disposable plate electrode, comprising:
- a. using screen printing technique to print at least one conductive film which comprises at least an anode electrode and a cathode electrode on a surface of a substrate and drying thereof at a temperature ranging from 40 to 120 degree Celsius;
b. using screen printing technique to print an electric insulating layer on the conductive film except selected areas which remain bare and are reserved for forming an anode coupling, a cathode coupling, a work electrode, a reference electrode, and a circular area for the biological active layer which consists of the work electrode and the reference electrode;
c. using screen printing technique to print a layer of cellulose carrier on the circular area and drying thereof at a room temperature ranging from 20 to 30 degree Celsius;
d. disposing an adhesive layer at the periphery of the circular area and adhering to thereon a layer of net protector for covering the biological active layer thereunder; and
e. dripping water solution which contains a biological active substance and a conductive mediator on the surface of the carrier and drying thereof in an ambience at a temperature ranging from 40 to 60 degree Celsius for completing the disposable plate electrode.
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Accused Products
Abstract
A manufacture method for forming a disposable plate electrode with biological active film is used to cooperate with a biological sensor for analyzing composition and measuring concentration of a test sample according to electric effect resulted from a biochemical reaction. The plate electrode comprises at least an electrode portion for transmission of the electric effect as well as a biological active film that reacts with the test sample chemically or biochemically. The biological active film contains a carrier layer (cellulose, for example) for adsorbing and keeping the biological active substance (enzyme, for example), which, the carrier layer, can change the electrode portion from hydrophobic into hydrophilic and protect the biological active substance against impairment during relatively higher temperature drying process. The method for forming a biological active film on the disposable electrode is mainly based on screen printing technique to form a conductive film, an electric insulating layer, a carrier layer, etc, for speedy production and low cost purpose.
9 Citations
14 Claims
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1. A manufacture method for forming a biological active layer on a disposable plate electrode, comprising:
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a. using screen printing technique to print at least one conductive film which comprises at least an anode electrode and a cathode electrode on a surface of a substrate and drying thereof at a temperature ranging from 40 to 120 degree Celsius;
b. using screen printing technique to print an electric insulating layer on the conductive film except selected areas which remain bare and are reserved for forming an anode coupling, a cathode coupling, a work electrode, a reference electrode, and a circular area for the biological active layer which consists of the work electrode and the reference electrode;
c. using screen printing technique to print a layer of cellulose carrier on the circular area and drying thereof at a room temperature ranging from 20 to 30 degree Celsius;
d. disposing an adhesive layer at the periphery of the circular area and adhering to thereon a layer of net protector for covering the biological active layer thereunder; and
e. dripping water solution which contains a biological active substance and a conductive mediator on the surface of the carrier and drying thereof in an ambience at a temperature ranging from 40 to 60 degree Celsius for completing the disposable plate electrode. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification