Electrospraying apparatus and method for coating particles

• US 6,746,869 B2
• Filed: 06/03/2002
• Issued: 06/08/2004
• Est. Priority Date: 06/12/1997
• Status: Expired due to Term

• Current Assignee: Beaz Company, LLC
• Original Assignee: Regents of The University of Minnesota (University of Minnesota)
• Inventors: Chen, Da-Ren, Pui, David Y. H.
• Primary Examiner(s): KETTER, JAMES S
• Application Number:
  US10/162,031
• Publication Number:
  US 20020150669A1
• Time in Patent Office: 736 Days
• Field of Search: 435/458, 435/459, 435/470, 435/471, 118/620, 118/303, 239/3, 239/690, 239/706
• US Class Current: 435/458
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: B01J 2/006 Coating of the granules without description of the process or the device by which the granules are obtained to prevent the granules sticking together B01J2/30
• CPC Class Codes: B01J 2/04 in a gaseous medium if combined with suspending the material in a gas, e.g. fluidised beds B01J2/16
• CPC Class Codes: B01J 2219/00371 comprising electrodes
• CPC Class Codes: B01J 2219/00436 Maskless processes
• CPC Class Codes: B05B 13/04 the spray heads being moved during spraying operation
• CPC Class Codes: B05B 5/025 Discharge apparatus, e.g. electrostatic spray guns
• CPC Class Codes: B05B 5/03 characterised by the use of gas , e.g. electrostatically assisted pneumatic spraying B05B5/04, B05B5/043, B05B5/047 take precedence
• CPC Class Codes: B05B 5/0533 Electrodes specially adapted therefor; Arrangements of electrodes
• CPC Class Codes: B05B 5/081 specially adapted for treating particulate materials
• CPC Class Codes: B05B 5/082 characterised by means for supporting, holding or conveying the objects
• CPC Class Codes: B05B 5/084 the objects lying on, or being supported above conveying means, e.g. conveyor belts
• CPC Class Codes: B05B 5/087 Arrangements of electrodes, e.g. of charging, shielding, collecting electrodes B05B5/12, B05B5/14 take precedence; arrangements of electrodes on the discharge apparatus B05B5/0533
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12N 15/8207 by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
• CPC Class Codes: C12N 15/87 Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
• CPC Class Codes: C12N 15/895 using biolistic methods

Abstract

An electrospraying apparatus and/or method is used to coat particles. For example, a flow including at least one liquid suspension may be provided through at least one opening at a spray dispenser end. The flow includes at least particles and a coating material. A spray of microdroplets suspending at least the particles is established forward of the spray dispenser end by creating a nonuniform electrical field between the spray dispenser end and an electrode electrically isolated therefrom. The particles are coated with at least a portion of the coating material as the microdroplet evaporates. For example, the suspension may include biological material particles.

Claims

1. A method of coating particles, the method comprising:

providing at least one opening at a spray dispenser end;

providing a flow comprising at least one liquid suspension through the at least one opening, wherein the flow comprising the at least one liquid suspension comprises at least particles and a coating material;

establishing from at least the one liquid suspension a spray of microdroplets suspending at least the particles forward of the spray dispenser end by creating a nonuniform electrical field between the spray dispenser end and an electrode electrically isolated therefrom, wherein the particles are coated with at least a portion of the coating material as the microdroplet evaporates.

2. The method of claim 1, wherein the particles comprise carrier particles and the coating material comprises biological material.

3. The method of claim 1, wherein the at least one suspension comprises biological material particles.

4. The method of claim 3, wherein the coating material comprises a facilitating transfer material.

5. The method of claim 1, wherein establishing a spray of microdroplets comprises concentrating electrical charge on the coated particles as the microdroplet evaporates, and further wherein a space charge effect of the concentrated electrical charge substantially prevents agglomeration of the coated particles.

6. The method of claim 5, wherein the electrical charge concentrated on a coated particle is in the range of about 80 percent to about 95 percent of a maximum charge that is held by the microdroplet.

7. The method of claim 1, wherein the particles have a nominal diameter of about 2 nanometers to about 1 micron.

8. The method of claim 7, wherein the particles have a nominal diameter of about 10 nanometers to about 100 nanometers.

9. The method of claim 1, wherein the microdroplets have a nominal diameter of about 10 nanometers to about 10 microns and the particles have a nominal diameter of about 2 nanometers to about 1 micron.

10. The method of claim 1, wherein establishing the spray of microdroplets comprises establishing a continuous spray of coated particles.

11. The method of claim 1, wherein providing at least one opening at a spray dispenser end comprises providing at least two openings at the spray dispenser end, and further wherein flowing at least one liquid suspension comprising particles and a coating material through the opening comprises:

flowing a liquid suspension comprising particles through a first opening at the spray dispenser end; and

flowing a liquid suspension comprising coating material through a second opening at the spray dispenser end.

12. The method of claim 1, wherein the method further comprises controlling the amount of coating material in the microdroplets of the spray.

13. A method for coating particles, the method comprising:

providing a first flow of a suspension comprising at least particles, wherein the particles have a nominal diameter of about 2 nanometers to about 1 micron;

providing a second flow of a solution comprising a coating material; and

establishing a spray of substantially dispersed coated particles comprising separated particles of the first flow encapsulated by coating material of the second flow, the substantially dispersed coated particles having an electrical charge applied thereto such that a space charge effect resulting from repulsion of the electrically charged substantially dispersed coated particles is used to prevent agglomeration of the one or more substantially dispersed coated particles of the spray.

14. The method of claim 13, wherein the particles have a nominal diameter of about 10 nanometers to about 100 nanometers.

15. The method of claim 13, wherein establishing the spray of substantially dispersed coated particles comprises dispensing a spray of microdroplets suspending one or more particles, and further wherein the one or more particles are coated with at least a portion of the coating material and the electrical charge is concentrated on the coated particles as the microdroplets evaporates.

16. The method of claim 15, wherein the method further comprises controlling the amount of coating material in the microdroplets of the spray.

17. The method of claim 15, wherein the electrical charge concentrated on a coated particle is in the range of about 80 percent to about 95 percent of a maximum charge that is held by the microdroplet.