Separation system, components of a separation system and methods of making and using them
First Claim
Patent Images
1. A chromatographic system comprising:
- a pumping system;
a column and detector array;
a collector system; and
a controller;
said column and detector array including a plurality of columns;
each of said columns containing a corresponding one of a plurality of size-compensated polymeric plugs;
each of said size-compensated polymeric plugs having the same characteristics, whereby said columns separate sample in a similar manner;
a source of solvent;
said pumping system being arrange to supply solvent from the source of solvent to the column and detector array under the control of the controller wherein effluent flows into the collector system from the column and detector array under the control of the controller;
said controller being connected to receive signals from detectors in the column and detector array indicating bands of solute and to activate the fraction collector accordingly.
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Abstract
Permeable polymeric monolithic materials are prepared in a column casing. In one embodiment, the permeable polymeric monolithic materials are polymerized while pressure is applied through a piston having a smooth piston head in contact with the polymerization mixture. The pressure eliminates wall effect and changes the structure in the column. Similarly, some columns that have a tendency to swell in the presence of aqueous solutions and pressurized while the solution is applied to prevent swelling and wall effect. This procedure also changes the structure in the column. The size of the separation effective openings can be controlled by the amount of the pressure and pores eliminated.
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Citations
21 Claims
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1. A chromatographic system comprising:
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a pumping system;
a column and detector array;
a collector system; and
a controller;
said column and detector array including a plurality of columns;
each of said columns containing a corresponding one of a plurality of size-compensated polymeric plugs;
each of said size-compensated polymeric plugs having the same characteristics, whereby said columns separate sample in a similar manner;
a source of solvent;
said pumping system being arrange to supply solvent from the source of solvent to the column and detector array under the control of the controller wherein effluent flows into the collector system from the column and detector array under the control of the controller;
said controller being connected to receive signals from detectors in the column and detector array indicating bands of solute and to activate the fraction collector accordingly. - View Dependent Claims (2)
said solvent is supplied to a pump array having a different one of a plurality of pumps in said pump array communicating with each a corresponding one of said plurality of columns in said column and detector array; and
a motor driven under the control of said controller to drive the array of pumps.
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3. A chromatographic system comprising:
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a sample injector;
at least one chromatographic column having a column casing and at least one permeable monolithic polymeric plug inside the column casing positioned to receive a sample from the sample injector;
a solvent system in communication with the chromatographic column for supplying a solvent to the at least one column, whereby the sample is separated into its components within the at least one column;
said column casing including a column casing wall with an internal column casing surface;
said at least one permeable monolithic polymeric plug with smooth walls without discontinuities in contact with an internal surface of the column walls; and
a utility device for receiving fluid from said column. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A separating system comprising:
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a permeable size-compensated polymeric support;
a sample injector positioned to inject sample onto the support of permeable size-compensated polymer;
a solvent system in communication with the support of permeable size-compensated polymer, whereby the sample is separated into its components within the separating system; and
a utility device positioned to receive fluid from said permeable size-compensated polymeric support. - View Dependent Claims (17, 18)
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19. A chromatographic system comprising:
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a sample injector;
at least one chromatographic column having a column casing and at least one permeable monolithic polymeric plug inside the column casing positioned to receive a sample from the sample injector;
a solvent system in communication with the chromatographic column for supplying a solvent to the at least one column, whereby the sample is separated into its components within the at least one column;
said column casing including a column casing wall with an internal column casing surface;
said at least one permeable monolithic polymeric plug having the structure shown in FIG. 5 when magnified 4.5 thousand times; and
a utility device for receiving fluid from said column. - View Dependent Claims (20)
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21. A separating system comprising:
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a permeable size-compensated polymeric support having the internal structure shown in FIG. 5 when magnified 9 thousand times;
a sample injector positioned to inject sample onto the support of permeable size-compensated polymer;
a solvent system in communication with the support of permeable size-compensated polymer, whereby the sample is separated into its components within the separating system; and
a utility device positioned to receive fluid from said permeable size-compensated polymeric support.
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Specification