Immunoassays involving surface enhanced Raman scattering
First Claim
1. A method for detecting the presence or amount of a target analyte in a sample comprising the steps of:
- (a) exposing the sample to a complex comprising an immobilized antibody capable of specifically binding the analyte, said antibody being specifically bound to a displacement agent, wherein said agent comprises;
(i) an analyte analog portion, capable of specifically binding the antibody, (ii) a label, which has surface enhanced Raman scattering (SERS) activity, or surface enhanced resonance Raman scattering (SERRS) activity, (iii) a SERRS or SERS surface-seeking group, whereby the analyte if present in the sample causes the displacement of the agent from the antibody;
(b) exposing the agent displaced from the antibody by the analyte to a SERRS or SERS surface such that said displaced agent becomes adsorbed on said surface; and
(c) detecting displaced agent associated with said surface using SERRS or SERS.
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Accused Products
Abstract
Immunoassay displacement methods for detecting (preferably identifying) the presence or amount of a target analyte (e.g. drug) in a sample comprising the steps of: (a) exposing the sample to a complex comprising an immobilized antibody capable of specifically binding the analyte, said antibody being specifically bound to a displacement agent, wherein said agent comprises: (I) an analyte analog capable of specifically binding the antibody, (ii) a surface enhanced resonance raman scattering (SERRS) or a surface enhanced raman scattering (SERS) active label, (iii) a SERRS or SERS surface-seeking group (e.g. benztriazole), whereby any analyte present in the sample causes the displacement of agent from the antibody; (b) exposing any displaced agent to a SERRS or SERS surface; and, (c) detecting any displaced agent associated with said surface using SERRS or SERS. In preferred forms of the invention several target analytes are detected from a single source or sample simultaneously. Also provided are agents per se e.g. for use in drug or explosive detection. These may include chromophores and linking groups.
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Citations
32 Claims
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1. A method for detecting the presence or amount of a target analyte in a sample comprising the steps of:
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(a) exposing the sample to a complex comprising an immobilized antibody capable of specifically binding the analyte, said antibody being specifically bound to a displacement agent, wherein said agent comprises;
(i) an analyte analog portion, capable of specifically binding the antibody, (ii) a label, which has surface enhanced Raman scattering (SERS) activity, or surface enhanced resonance Raman scattering (SERRS) activity, (iii) a SERRS or SERS surface-seeking group, whereby the analyte if present in the sample causes the displacement of the agent from the antibody; (b) exposing the agent displaced from the antibody by the analyte to a SERRS or SERS surface such that said displaced agent becomes adsorbed on said surface; and
(c) detecting displaced agent associated with said surface using SERRS or SERS. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification