Pathogen-resistant plants transformed with a DNA encoding sarcotoxin 1A linked to a signal peptide and a method for production thereof
First Claim
Patent Images
1. A method of conferring resistance to pathogenic fungi on a plant, wherein the method comprises the steps of:
- transforming a plant cell with an expression vector, wherein said expression vector comprises;
an expression cassette comprising a first plant promoter induced by stress operably linked to a DNA sequence encoding Sarcotoxin 1a, wherein a DNA sequence encoding a signal peptide is operatively positioned between the first plant promoter and the DNA sequence encoding Sarcotoxin 1a; and
a constitutively expressed plant promoter positioned adjacent to the first plant promoter; and
regenerating the plant cell into a transgenic plant, wherein the transgenic plant has enhanced resistance to pathogenic fungi as compared to a corresponding untransformed plant.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention is drawn to a method of conferring resistance to pathogenic fungi on a plant by transformation with a nucleic acid encoding Sarcotoxin 1a operatively linked to a signal peptide, and optionally linked via the hinge region of tobacco chitinase to a plant peptide, and plants thereby obtained.
-
Citations
16 Claims
-
1. A method of conferring resistance to pathogenic fungi on a plant, wherein the method comprises the steps of:
-
transforming a plant cell with an expression vector, wherein said expression vector comprises;
an expression cassette comprising a first plant promoter induced by stress operably linked to a DNA sequence encoding Sarcotoxin 1a, wherein a DNA sequence encoding a signal peptide is operatively positioned between the first plant promoter and the DNA sequence encoding Sarcotoxin 1a; and
a constitutively expressed plant promoter positioned adjacent to the first plant promoter; and
regenerating the plant cell into a transgenic plant, wherein the transgenic plant has enhanced resistance to pathogenic fungi as compared to a corresponding untransformed plant. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
-
-
9. A transgenic plant which is resistant to pathogenic fungi, wherein the plant comprises an expression vector, wherein the expression vector comprises:
-
a first expression cassette comprising a DNA sequence encoding Sarcotoxin 1a operably linked to a promoter induced by stress, wherein a DNA sequence encoding a signal peptide is operatively positioned between the promoter induced by stress and the DNA sequence encoding Sarcotoxin 1a; and
a second expression cassette comprising a drug resistance gene operably linked to a constitutively expressed promoter;
wherein the first and second expression cassettes are positioned adjacent to each other, and wherein the transgenic plant has enhanced resistance to pathogenic fungi as compared to a corresponding untransformed plant. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
-
Specification