Imaging systems for signals on a surface

• US 6,750,963 B2
• Filed: 05/21/2002
• Issued: 06/15/2004
• Est. Priority Date: 05/21/2002
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Sampas, Nicholas M.
• Primary Examiner(s): EVANS, FANNIE L
• Application Number:
  US10/153,128
• Publication Number:
  US 20030218746A1
• Time in Patent Office: 756 Days
• Field of Search: 356/317, 356/318, 356/417, 250/458.1, 250/459.1, 250/461.1, 250/461.2, 436/172
• US Class Current: 356/318
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

Apparatus and methods are disclosed for imaging surfaces that comprise a sample. One embodiment of the present invention is an imaging apparatus comprising a holder for a surface, a light source adapted to illuminate the surface, a diffractive element between the holder and the light source and an imaging detector adapted to receive light from the surface. In a method for imaging a surface comprising a plurality of discrete features, light is selectively diffracted on to one or more predetermined features of the surface at a predetermined point in time. This step is repeated until substantially all of the features on the surface are illuminated. Light is detected from the surface to thereby image the surface.

Claims

1. An imaging apparatus comprising:

(a) a holder for a surface,

(b) a light source adapted to illuminate said surface,

(c) a diffractive element between said holder and said light source, said diffractive element comprising an array of diffraction gratings wherein said diffractive element is arranged to sequentially illuminate different sets of features on said surface having a sample deposited thereon until substantially all of the features on said surface are illuminated and

(d) an imaging detector adapted to receive light from said surface.

2. An apparatus according to claim 1 wherein said light source is a laser, a focused lamp or an LED.

3. An apparatus according to claim 1 wherein said diffractive element is a spinning disk, a rotating drum or a rectilinear array.

4. An apparatus according to claim 1 wherein said diffractive element is synchronized to a said detector.

5. An apparatus according to claim 1 wherein said diffractive element is non-synchronized to said detector.

6. An apparatus according to claim 1 wherein said imaging detector is a solid state detector.

7. An apparatus according to claim 6 wherein said solid state detector is a CCD array, a CMOS array, a diode array or a PIN-diode array.

8. An apparatus according to claim 1 further comprising a focusing element.

9. An apparatus according to claim 1 further comprising an objective lens.

10. An apparatus according to claim 1 wherein said diffractive element is movable.

11. An apparatus according to claim 10 wherein said diffractive element is driven by a motor.

12. A method for imaging a surface comprising a plurality of discrete features, said method comprising:

(a) selectively diffracting light on to a set of one or more predetermined features of said surface at a predetermined point in time and detecting light from said surface,

(b) repeating step (a) for different sets of one or more predetermined features until substantially all of the features on said surface are illuminated to thereby image said surface.

13. A method according to claim 12 wherein said light detected is fluorescence.

14. A method according to claim 12 wherein said discrete features are polynucleotides.

15. A method according to claim 12, which comprises selectively directing light from a light source on to regions of a diffractive element disposed with respect to said surface such that one or more predetermined features of said surface are illuminated at a predetermined point in time wherein said diffractive element comprises an array of diffraction gratings and wherein said diffractive element is arranged to illuminate a set of separate pixel regions of a sample on the surface at one predetermined point in time and to illuminate another set of different pixel regions of said sample at another predetermined point in time.

16. A method according to claim 15 wherein said diffractive element is a disk, drum or rectilinear array.

17. A method according to claim 15 wherein said diffractive element is driven by a motor.

18. A method according to claim 15 wherein said selectively directing light comprises moving said diffractive element with respect to a stationary light source.

19. A method according to claim 15 wherein said selectively directing light comprises moving said light source with respect to a stationary diffractive element.

20. A method according to claim 12 wherein said detecting is carried out using a solid state detector.

21. An imaging apparatus comprising:

(a) a holder for a surface,

(b) an array of light sources adapted to illuminate said surface,

(c) a diffractive element between said holder and said light sources, said diffractive element comprising an array of openings and

(d) an imaging detector adapted to receive light from said surface.